Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section.

Autor: Lai A; Leni and Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA., Gansau J; Leni and Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA., Gullbrand SE; McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania USA., Crowley J; Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School University of New South Wales Sydney Australia., Cunha C; i3S-Instituto de Investigação e InovaçãoemSaúde Universidade do Porto Porto Portugal., Dudli S; University Clinic of Rheumatology, Center of Experimental Rheumatology Balgrist University Hospital, University of Zurich Zurich Switzerland., Engiles JB; Department of Pathobiology, New Bolton Center, School of Veterinary Medicine University of Pennsylvania Kennett Square Pennsylvania USA., Fusellier M; Regenerative Medicine and Skeleton, Inserm, UMR 1229, RMeS Université de Nantes, ONIRIS Nantes Cedex France., Goncalves RM; i3S-Instituto de Investigação e InovaçãoemSaúde Universidade do Porto Porto Portugal.; Instituto de CiênciasBiomédicas Abel Salazar Universidade do Porto Porto Portugal., Nakashima D; Department of Orthopaedic Surgery Keio University School of Medicine Tokyo Japan., Okewunmi J; Leni and Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA., Pelletier M; Surgical and Orthopaedic Research Laboratories, Prince of Wales Clinical School University of New South Wales Sydney Australia., Presciutti SM; Department of Orthopaedic Surgery Emory University Atlanta Georgia USA., Schol J; Department of Orthopaedic Surgery, Surgical Science Tokai University School of Medicine Isehara Japan., Takeoka Y; Department of Orthopaedic Surgery Brigham and Women's Hospital Boston Massachusetts USA., Yang S; Department of Spinal Surgery The Third Hospital of Hebei Medical University Shijiazhuang China., Yurube T; Department of Orthopaedic Surgery Kobe University Graduate School of Medicine Kobe Japan., Zhang Y; McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania USA., Iatridis JC; Leni and Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.
Jazyk: angličtina
Zdroj: JOR spine [JOR Spine] 2021 May 26; Vol. 4 (2), pp. e1150. Date of Electronic Publication: 2021 May 26 (Print Publication: 2021).
DOI: 10.1002/jsp2.1150
Abstrakt: Background: Rats are a widely accepted preclinical model for evaluating intervertebral disc (IVD) degeneration and regeneration. IVD morphology is commonly assessed using histology, which forms the foundation for quantifying the state of IVD degeneration. IVD degeneration severity is evaluated using different grading systems that focus on distinct degenerative features. A standard grading system would facilitate more accurate comparison across laboratories and more robust comparisons of different models and interventions.
Aims: This study aimed to develop a histology grading system to quantify IVD degeneration for different rat models.
Materials & Methods: This study involved a literature review, a survey of experts in the field, and a validation study using 25 slides that were scored by 15 graders from different international institutes to determine inter- and intra-rater reliability.
Results: A new IVD degeneration grading system was established and it consists of eight significant degenerative features, including nucleus pulposus (NP) shape, NP area, NP cell number, NP cell morphology, annulus fibrosus (AF) lamellar organization, AF tears/fissures/disruptions, NP-AF border appearance, as well as endplate disruptions/microfractures and osteophyte/ossification. The validation study indicated this system was easily adopted, and able to discern different severities of degenerative changes from different rat IVD degeneration models with high reproducibility for both experienced and inexperienced graders. In addition, a widely-accepted protocol for histological preparation of rat IVD samples based on the survey findings include paraffin embedding, sagittal orientation, section thickness < 10 μm, and staining using H&E and/or SO/FG to facilitate comparison across laboratories.
Conclusion: The proposed histological preparation protocol and grading system provide a platform for more precise comparisons and more robust evaluation of rat IVD degeneration models and interventions across laboratories.
Competing Interests: The authors declare no conflicts of interest.
(© 2021 The Authors. JOR Spine published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.)
Databáze: MEDLINE
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