Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements.

Autor:	Zimmer WE; Department of Medical Physiology, Texas A&M College of Medicine, Bryan, TX, USA. wezimmer@tamu.edu., Schwartz RJ; Department of Biology & Biochemistry, University of Houston, Houston, TX, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2319, pp. 119-136.
DOI:	10.1007/978-1-0716-1480-8_14
Abstrakt:	We describe a novel, efficient method to identify cis-acting DNA sequences that drive cell-specific gene expression during development. We utilize transfer of Bacterial Artificial Chromosome (BAC) genomic DNAs, modified to contain a reporter gene, into fertilized mouse embryos and placing the injected embryos into pseudopregnant recipient females. The embryos are allowed to develop in utero for defined times after which they are collected for analysis. Using DNAs containing the LacZ reporter gene facilitates the analysis of gene activity through microscopy of intact embryos and subsequent sectioning of the stained embryos. With this technique cis-element activity can be identified and evaluated through further mutational analysis of the injected BAC DNA. This allows the identification of important gene regulatory domains that specify stage-specific gene expression in the developing embryo. (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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