Reliability of carbapenem inactivation method (CIM) and modified carbapenem inactivation method (mCIM) for detection of OXA-48-like and NDM-1.

Autor: Rizvi M; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman. Electronic address: rizvimeher@squ.edu.om., Sami H; Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India., Azam M; Department of Medical Laboratories, College of Applied Medical Science, Qassim University, Buraydah, Saudi Arabia., Ben Khalid D; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman., Al Jabri Z; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman. Electronic address: zaeema@squ.edu.om., Khan F; Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India., Sultan A; Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India., Singh A; Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India., Perween N; Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India., Al Quraini M; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman. Electronic address: munquraini@outlook.com., Al Muharrmi Z; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman. Electronic address: almuharrmi@gmail.com., Rizvi SG; Department of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University Hospital, Muscat, Oman. Electronic address: rizvi@squ.edu.om.
Jazyk: angličtina
Zdroj: Indian journal of medical microbiology [Indian J Med Microbiol] 2021 Oct-Dec; Vol. 39 (4), pp. 451-456. Date of Electronic Publication: 2021 Jul 28.
DOI: 10.1016/j.ijmmb.2021.07.004
Abstrakt: Purpose: Carbapenem inactivation method (CIM) and modified carbapenem inactivation method (mCIM) were recently developed for rapid detection of carbapenemase producing Gram negative bacilli (CP-GNB). In this study we compared the ability of modified Hodge test (MHT), CIM and mCIM to identify CP-GNB in Oman and India.
Methods: Fifty fully characterized and genotyped CP-GNB (26 OXA-48-like, 2 NDM-1 from Oman and 22 NDM-1 from India) and 8 AmpC as controls in India were subjected to MHT, CIM, mCIM and mCIM with in-house modifications. Wilcoxon paired test and receiver operating characteristics (ROC) were utilised for statistical analysis.
Results: Isolates were predominantly OXA-48-like genes producing Klebsiella pneumoniae from Oman and NDM-1 producing Escherichia coli from India. MHT was positive in all except one OXA-48-like producers and in 70.8 ​% of the NDM-1 isolates. The sensitivity of CIM in detecting 0XA-48 like and NDM-1 carbapenemases were 39.2% and 87.5% respectively. mCIM at 4 ​h detected 92.3 ​% and 79.1% of 0XA-48 and NDM-1 respectively. Using receiver operative characteristics (ROC), highest sensitivity and specificity for detection of OXA-48-like was obtained by mCIM at 4 ​h at cut off 17 ​mm while for NDM-1 CIM was the test of choice at 16 ​mm.
Conclusion: CIM and mCIM are simple, cheap and easy tests to perform. CIM gave excellent results with NDM1 strains while it was quite poor in predicting OXA-48-like. We recommend CIM and eCIM for rapid identification of NDM-1 producers and mCIM at 4 ​h and MHT for detection of OXA-48-like. No one method can correctly detect both genotypes. As determined by ROC curves a zone of inhibition of 17 ​mm was considered adequate for detection of OXA-48-like and 16 ​mm of NDM-1 by mCIM at 4 ​h and CIM respectively.
(Copyright © 2021 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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