Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69.
| Autor: | Dos Santos CA; Instituto Adolfo Lutz, São Paulo, Brazil., Hernandes RT; Instituto de Biociências de Botucatu, Universidade Estadual Paulista 'Júlio de Mesquita Filho', Botucatu, Brazil., Cunha MPV; Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Nagamori FO; Instituto Adolfo Lutz, São Paulo, Brazil., Gonçalves CR; Instituto Adolfo Lutz, São Paulo, Brazil., Sacchi CT; Instituto Adolfo Lutz, São Paulo, Brazil., Tiba-Casas MR; Instituto Adolfo Lutz, São Paulo, Brazil., Camargo CH; Instituto Adolfo Lutz, São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Chemotherapy [Chemotherapy] 2021; Vol. 66 (3), pp. 92-98. Date of Electronic Publication: 2021 Jul 29. |
| DOI: | 10.1159/000517817 |
| Abstrakt: | Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen. (© 2021 S. Karger AG, Basel.) |
| Databáze: | MEDLINE |
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