A Novel Ruthenium(II) Complex With Lapachol Induces G2/M Phase Arrest Through Aurora-B Kinase Down-Regulation and ROS-Mediated Apoptosis in Human Prostate Adenocarcinoma Cells.

Autor: De Grandis RA; School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Brazil.; School of Medicine, University of Araraquara, Araraquara, Brazil., Oliveira KM; Department of Chemistry, Federal University of São Carlos, São Carlos, Brazil., Guedes APM; Department of Chemistry, Federal University of São Carlos, São Carlos, Brazil., Dos Santos PWS; School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Aissa AF; Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago College of Medicine, Chicago, IL, United States., Batista AA; Department of Chemistry, Federal University of São Carlos, São Carlos, Brazil., Pavan FR; School of Pharmaceutical Sciences, São Paulo State University, Araraquara, Brazil.
Jazyk: angličtina
Zdroj: Frontiers in oncology [Front Oncol] 2021 Jun 24; Vol. 11, pp. 682968. Date of Electronic Publication: 2021 Jun 24 (Print Publication: 2021).
DOI: 10.3389/fonc.2021.682968
Abstrakt: Lapachol is a well-studied natural product that has been receiving great interest due to its anticancer properties that target oxidative stress. In the present work, two novel lapachol-containing ruthenium(II) complexes [Ru(Lap)(dppm)(bipy)]PF 6 ( 1 ) and [Ru(Lap)(dppm)(phen)]PF 6 ( 2 ) [Lap = lapachol, dppm = 1,1'-bis(diphosphino)methane, bipy = 2,2'-bipyridine, phen = 1,10-phenantroline] were synthesized, fully characterized, and investigated for their cellular and molecular responses on cancer cell lines. We found that both complexes exhibited a potent cytotoxic effect in a panel of cancer cell lines in monolayer cultures, as well as in a 3D model of multicellular spheroids formed from DU-145 human prostate adenocarcinoma cells. Furthermore, the complex ( 2 ) suppressed the colony formation, induced G2/M-phase arrest, and downregulated Aurora-B. The mechanism studies suggest that complex ( 2 ) stimulate the overproduction of reactive oxygen species (ROS) and triggers caspase-dependent apoptosis as a result of changes in expression of several genes related to cell proliferation and caspase-3 and -9 activation. Interestingly, we found that N-acetyl-L-cysteine, a ROS scavenger, suppressed the generation of intracellular ROS induced by complex ( 2 ), and decreased its cytotoxicity, indicating that ROS-mediated DNA damage leads the DU-145 cells into apoptosis. Overall, we highlighted that coordination of lapachol to phosphinic ruthenium(II) compounds considerably improves the antiproliferative activities of resulting complexes granting attractive selectivity to human prostate adenocarcinoma cells. The DNA damage response to ROS seems to be involved in the induction of caspase-mediated cell death that plays an important role in the complexes' cytotoxicity. Upon further investigations, this novel class of lapachol-containing ruthenium(II) complexes might indicate promising chemotherapeutic agents for prostate cancer therapy.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 De Grandis, Oliveira, Guedes, dos Santos, Aissa, Batista and Pavan.)
Databáze: MEDLINE
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