Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020.

Autor: Liyaskina EV; Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia., Rakova NA; Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia., Kitykina AA; Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia., Rusyaeva VV; Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia., Toukach PV; N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Fomenkov A; New England Biolabs Inc., Ipswich, MA, United States of America., Vainauskas S; New England Biolabs Inc., Ipswich, MA, United States of America., Roberts RJ; New England Biolabs Inc., Ipswich, MA, United States of America., Revin VV; Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Jul 06; Vol. 16 (7), pp. e0253482. Date of Electronic Publication: 2021 Jul 06 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0253482
Abstrakt: Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.
Competing Interests: The authors have read the journal’s policy and have the following competing interests: A.F, S.V. and R.J.R. are paid employees of New England Biolabs (https://www.neb.com/). There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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