Prospection of plasma proteins as biomarkers for diabetes mellitus monitoring.

Autor: de Paula Silva L; Graduate Program in Pharmaceutical Science, Department of Clinical Analysis, Federal University of Paraná, Rua Prefeito Lothário Meissner, 632, Curitiba, PR 80210-170 Brazil., de Moraes Rego FG; Graduate Program in Pharmaceutical Science, Department of Clinical Analysis, Federal University of Paraná, Rua Prefeito Lothário Meissner, 632, Curitiba, PR 80210-170 Brazil., Picheth G; Graduate Program in Pharmaceutical Science, Department of Clinical Analysis, Federal University of Paraná, Rua Prefeito Lothário Meissner, 632, Curitiba, PR 80210-170 Brazil., Müller-Santos M; Graduate Program in Sciences - Biochemistry, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR Brazil., Alberton D; Graduate Program in Pharmaceutical Science, Department of Clinical Analysis, Federal University of Paraná, Rua Prefeito Lothário Meissner, 632, Curitiba, PR 80210-170 Brazil.
Jazyk: angličtina
Zdroj: Journal of diabetes and metabolic disorders [J Diabetes Metab Disord] 2021 Mar 29; Vol. 20 (1), pp. 611-620. Date of Electronic Publication: 2021 Mar 29 (Print Publication: 2021).
DOI: 10.1007/s40200-021-00788-1
Abstrakt: Purpose: This prospective study aimed to detect and identify plasma proteins differentially expressed between groups of Brazilian diagnosed with type 1 (T1DM), type 2 (T2DM) diabetes with good and poor glycemic control and the non-diabetic group denominated control group (CG).
Methods: Patients with T1DM and T2DM were subdivided according to their glycated haemoglobin (HbA 1c ) level: ≥ 53 mmol/mol and < 53 mmol/mol. Each subgroup was composed of ten subjects ( n  = 10). The plasma from each subgroup was pooled and depleted of albumin and IgG. The reminiscent proteins were quantified and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The relative volume of protein bands was determined by densitometry analysis, and those with differential abundance were identified by MALDI-TOF mass spectrometry.
Results: Alpha 2 - Macroglobulin (AMG) was 1.3-fold more abundant in T1DM with HbA 1c  ≥ 53 mmol/mol and < 53 mmol/mol and 1.4-fold more abundant in T2DM with HbA 1c  ≥ 53 mmol/mol compared to CG. Ceruloplasmin (Cp) and Haptoglobin (Hp) were overexpressed above 1.5-fold in all DM subgroups. Cp in T1DM and Hp in both types of DM were more expressed in HbA 1c  ≥ 53 mmol/mol than <53 mmol/mol. Apolipoprotein A-I (Apo A-I) was upregulated only in T2DM subgroups.
Conclusion: In summary, three positive acute-phase proteins, AMG, Cp and Hp were more abundant in diabetic individuals regardless of the diabetes type. The highest Hp abundance in both types of DM with HbA 1c  ≥ 53 mmol/mol, reinforces Hp as a possible biomarker associated with diabetic complications.
Competing Interests: Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest.
(© Springer Nature Switzerland AG 2021.)
Databáze: MEDLINE
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