| Autor: |
García-García JD; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Anguiano-Cabello JC; Health Sciences Areas, La Salle Saltillo University, Saltillo 25298, Coahuila, Mexico., Arredondo-Valdés R; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Candido Del Toro CA; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Martínez-Hernández JL; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Segura-Ceniceros EP; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Govea-Salas M; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., González-Chávez ML; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Ramos-González R; CONACYT-Faculty of Chemistry Autonomous, University of Coahuila, Saltillo 25280, Coahuila, Mexico., Esparza-González SC; Odontology Faculty, Autonomous University of Coahuila, Saltillo 25125, Coahuila, Mexico., Ascacio-Valdés JA; Department of Food Research, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., López-Badillo CM; Ceramic Materials Academic Group of the Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico., Ilyina A; Nanobioscience Group, Faculty of Chemistry, Autonomous University of Coahuila, Saltillo 25280, Coahuila, Mexico. |
| Abstrakt: |
In Mexico, mistletoes have several applications in traditional medicine due to the great variety of compounds with biological activities that have not been characterized to date. The goals of the present study are to analyze the composition of minerals and phytochemical compounds in Mexican mistletoes Phoradendron bollanum and Viscum album subs. austriacum qualitatively and quantitatively, identify the compounds using HPLC-MS, and assess the antimicrobial potential in phytopathogenic microorganism control. Mineral content was evaluated with X-ray fluorescence. Three types of extracts were prepared: ethanol, water, and aqueous 150 mM sodium chloride solution. Characterization was carried out using qualitative tests for phytochemical compound groups, analytical methods for proteins, reducing sugars, total phenol, flavonoids quantification, and HPLC-MS for compound identification. The antimicrobial activity of mistletoe's liquid extracts was evaluated by microplate assay. K and Ca minerals were observed in both mistletoes. A qualitative test demonstrated alkaloids, carbohydrates, saponins, flavonoids, tannins, and quinones. Ethanolic extract showed flavonoids, 3845 ± 69 and 3067 ± 17.2 mg QE/g for Phoradendron bollanum and Viscum album subs. austriacum , respectively, while aqueous extracts showed a total phenol content of 65 ± 6.9 and 90 ± 1.19 mg GAE/g Phoradendron bollanum and Viscum album subs. austriacum , respectively. HPLC-MS identified largely hydroxycinnamic acids and methoxycinnamic acids. Clavibacter michiganenses was successfully inhibited by aqueous extract of both mistletoes. |
