Fibrin Glue Implants Seeded with Dental Pulp and Periodontal Ligament Stem Cells for the Repair of Periodontal Bone Defects: A Preclinical Study.

Autor: Enukashvily NI; Cell Technologies Lab, North-Western State Medical University, 191015 St. Petersburg, Russia.; Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia., Dombrovskaya JA; General Dentistry Department, North-Western State Medical University, 191015 St. Petersburg, Russia., Kotova AV; Cell Technologies Lab, North-Western State Medical University, 191015 St. Petersburg, Russia.; Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia., Semenova N; Russian Research Institute of Hematology and Transfusiology, FMBA of Russia, 191024 St. Petersburg, Russia., Karabak I; Children's Scientific and Clinical Center for Infectious Diseases, 197022 St. Petersburg, Russia., Banashkov RE; X-ray Centers «Picasso», 191123 St. Petersburg, Russia., Baram D; Russian Research Institute of Hematology and Transfusiology, FMBA of Russia, 191024 St. Petersburg, Russia., Paderina T; General Dentistry Department, North-Western State Medical University, 191015 St. Petersburg, Russia., Bilyk SS; Vreden National Medical Research Center of Traumatology and Orthopedics, 195427 St. Petersburg, Russia., Grimm WD; Cell Technologies Lab, North-Western State Medical University, 191015 St. Petersburg, Russia.; Periodontology, Faculty of Health, School of Dental Medicine, Witten/Herdecke University, 58455 Witten, Germany., Kovalenko AN; Vreden National Medical Research Center of Traumatology and Orthopedics, 195427 St. Petersburg, Russia., Ivolgin D; Cell Technologies Lab, North-Western State Medical University, 191015 St. Petersburg, Russia.; Cell Technologies Center Pokrovsky, LLC, 199106 St. Petersburg, Russia., Prikhodko EM; Cell Technologies Center Pokrovsky, LLC, 199106 St. Petersburg, Russia.; Therapeutic Department, North-Western State Medical University, 191015 St. Petersburg, Russia., Silin AV; General Dentistry Department, North-Western State Medical University, 191015 St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2021 Jun 01; Vol. 8 (6). Date of Electronic Publication: 2021 Jun 01.
DOI: 10.3390/bioengineering8060075
Abstrakt: A technology to create a cell-seeded fibrin-based implant matching the size and shape of bone defect is required to create an anatomical implant. The aim of the study was to develop a technology of cell-seeded fibrin gel implant creation that has the same shape and size as the bone defect at the site of implantation. Using computed tomography (CT) images, molds representing bone defects were created by 3D printing. The form was filled with fibrin glue and human dental pulp stem cells (DPSC). The viability, set of surface markers and osteogenic differentiation of DPSC grown in fibrin gel along with the clot retraction time were evaluated. In mice, an alveolar bone defect was created. The defect was filled with fibrin gel seeded with mouse DPSC. After 28 days, the bone repair was analyzed with cone beam CT and by histological examination. The proliferation rate, set of surface antigens and osteogenic potential of cells grown inside the scaffold and in 2D conditions did not differ. In mice, both cell-free and mouse DPSC-seeded implants increased the bone tissue volume and vascularization. In mice with cell-seeded gel implants, the bone remodeling process was more prominent than in animals with a cell-free implant. The technology of 3D-printed forms for molding implants can be used to prepare implants using components that are not suitable for 3D printing.
Databáze: MEDLINE
Externí odkaz: