CALM supports clathrin-coated vesicle completion upon membrane tension increase.

Autor:	Willy NM; Department of Physics, The Ohio State University, Columbus, OH 43210., Colombo F; College of Pharmacy, The Ohio State University, Columbus, OH 43210., Huber S; Department of Physics, The Ohio State University, Columbus, OH 43210., Smith AC; College of Pharmacy, The Ohio State University, Columbus, OH 43210., Norton EG; College of Pharmacy, The Ohio State University, Columbus, OH 43210., Kural C; Department of Physics, The Ohio State University, Columbus, OH 43210; kural.1@osu.edu cocucci.1@osu.edu.; Interdisciplinary Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210., Cocucci E; College of Pharmacy, The Ohio State University, Columbus, OH 43210; kural.1@osu.edu cocucci.1@osu.edu.; Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Jun 22; Vol. 118 (25).
DOI:	10.1073/pnas.2010438118
Abstrakt:	The most represented components of clathrin-coated vesicles (CCVs) are clathrin triskelia and the adaptors clathrin assembly lymphoid myeloid leukemia protein (CALM) and the heterotetrameric complex AP2. Investigation of the dynamics of AP180-amino-terminal-homology (ANTH) recruitment during CCV formation has been hampered by CALM toxicity upon overexpression. We used knock-in gene editing to express a C-terminal-attached fluorescent version of CALM, while preserving its endogenous expression levels, and cutting-edge live-cell microscopy approaches to study CALM recruitment at forming CCVs. Our results demonstrate that CALM promotes vesicle completion upon membrane tension increase as a function of the amount of this adaptor present. Since the expression of adaptors, including CALM, differs among cells, our data support a model in which the efficiency of clathrin-mediated endocytosis is tissue specific and explain why CALM is essential during embryogenesis and red blood cell development. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
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