Plant "helper" immune receptors are Ca 2+ -permeable nonselective cation channels.

Autor: Jacob P; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Kim NH; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Wu F; Department of Biology, Duke University, Durham, NC 27708, USA.; Department of Horticulture, Foshan University, Foshan, China., El-Kasmi F; Department of Plant Physiology, Centre of Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany., Chi Y; Department of Biology, Duke University, Durham, NC 27708, USA., Walton WG; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Furzer OJ; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Lietzan AD; Division of Oral and Craniofacial Health Sciences, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Sunil S; Department of Plant Physiology, Centre of Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany., Kempthorn K; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Redinbo MR; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Pei ZM; Department of Biology, Duke University, Durham, NC 27708, USA. zpei@duke.edu lwan@cemps.ac.cn dangl@email.unc.edu., Wan L; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. zpei@duke.edu lwan@cemps.ac.cn dangl@email.unc.edu.; National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China., Dangl JL; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. zpei@duke.edu lwan@cemps.ac.cn dangl@email.unc.edu.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2021 Jul 23; Vol. 373 (6553), pp. 420-425. Date of Electronic Publication: 2021 Jun 17.
DOI: 10.1126/science.abg7917
Abstrakt: Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis , a subfamily of "helper" NLRs is required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca 2+ ) influx in plant and human cells. NRG1.1-dependent Ca 2+ influx and cell death were sensitive to Ca 2+ channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca 2+ influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that Arabidopsis helper NLRs form Ca 2+ -permeable cation channels to directly regulate cytoplasmic Ca 2+ levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly.
(Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze: MEDLINE
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