COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR.

Autor:	Voon K; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia., Johari NA; Institute for Research, Development and Innovation (IRDI), International Medical University, Kuala Lumpur, Malaysia., Lim KL; Institute for Research, Development and Innovation (IRDI), International Medical University, Kuala Lumpur, Malaysia., Wong ST; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia., Khaw LT; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia., Wong SF; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.; Centre for Environmental and Population Health Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur, Malaysia., Chan EWL; Institute for Research, Development and Innovation (IRDI), International Medical University, Kuala Lumpur, Malaysia., Chan KK; Human Biology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia., Tan BK; Human Biology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia., Ramzi NH; Institute for Research, Development and Innovation (IRDI), International Medical University, Kuala Lumpur, Malaysia., Lim PKC; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.; Institute for Research, Development and Innovation (IRDI), International Medical University, Kuala Lumpur, Malaysia., Sulaiman LH; Department of Community Medicine, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.; Centre for Environmental and Population Health Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur, Malaysia.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2021 May 05; Vol. 11 (9), pp. e4005. Date of Electronic Publication: 2021 May 05 (Print Publication: 2021).
DOI:	10.21769/BioProtoc.4005
Abstrakt:	The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-developed countries with limited resources. Here, we propose a simple, reliable pooling strategy for COVID-19 testing using clinical nasopharyngeal (NP) and/or oropharyngeal (OP) swabs. The strategy includes the pooling of 10 NP/OP swabs for extraction and subsequent testing via quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR), and may also be applied to the screening of other pathogens. Competing Interests: Competing interestsThe author(s) declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. While performing this research, no company had invested in the research and no commercialization was intended. (Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.)
Databáze:	MEDLINE
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