Detection of Anti-Nucleocapsid Antibody in COVID-19 Patients in Bangladesh Is not Correlated with Previous Dengue Infection.

Autor:	Lytton SD; SeraDiaLogistics, 81545 Munich, Germany., Yeasmin M; National Institute of Laboratory Medicine and Referral Center, Sher E-Bangla Nagar, Dhaka 1207, Bangladesh., Ghosh AK; Dhaka Medical College Hospital, Dhaka 1000, Bangladesh., Bulbul MRH; Institute for Developing Science and Health Initiatives, Mohakhali, Dhaka 1212, Bangladesh., Molla MMA; National Institute of Laboratory Medicine and Referral Center, Sher E-Bangla Nagar, Dhaka 1207, Bangladesh., Herr M; NovaTec Immundiagnostica GmbH, 63128 Dietzenbach, Germany., Duchmann H; NovaTec Immundiagnostica GmbH, 63128 Dietzenbach, Germany., Sharif MM; Dhaka Medical College Hospital, Dhaka 1000, Bangladesh., Nafisa T; National Institute of Laboratory Medicine and Referral Center, Sher E-Bangla Nagar, Dhaka 1207, Bangladesh., Amin MR; Dhaka Medical College Hospital, Dhaka 1000, Bangladesh., Hosen N; National Institute of Laboratory Medicine and Referral Center, Sher E-Bangla Nagar, Dhaka 1207, Bangladesh., Rahman MT; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Islam S; Bangladesh Medical College and Hospital, 14/A Dhanmondi, Dhaka 1209, Bangladesh., Islam A; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Shamsuzzaman AKM; National Institute of Laboratory Medicine and Referral Center, Sher E-Bangla Nagar, Dhaka 1207, Bangladesh.
Jazyk:	angličtina
Zdroj:	Pathogens (Basel, Switzerland) [Pathogens] 2021 May 22; Vol. 10 (6). Date of Electronic Publication: 2021 May 22.
DOI:	10.3390/pathogens10060637
Abstrakt:	Background: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh. Methods: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA. Results: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9-23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27-30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations. Conclusions: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu