PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII.

Autor:	Reddy MR; Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Mangrauthia SK; Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Reddy SV; Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India., Manimaran P; Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India., Yugandhar P; Physiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Babu PN; Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Vishnukiran T; Physiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Subrahmanyam D; Physiology Laboratory, Crop Production Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Sundaram RM; Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India., Balachandran SM; Biotechnology Laboratory, Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad 500030, India.
Jazyk:	angličtina
Zdroj:	Journal of advanced research [J Adv Res] 2020 Nov 23; Vol. 30, pp. 197-211. Date of Electronic Publication: 2020 Nov 23 (Print Publication: 2021).
DOI:	10.1016/j.jare.2020.11.008
Abstrakt:	Introduction: Photosystem II (PSII) protein complex plays an essential role in the entire photosynthesis process. Various known and unknown protein factors are involved in the dynamics of the PSII complex that need to be characterized in crop plants for enhancing photosynthesis efficiency and productivity. Objectives: The experiments were conducted to decipher the regulatory proteins involved in PSII dynamics of rice crop. Methods: A novel rice regulatory protein PAP90 (PSII auxiliary protein ~90 kDa) was characterized by generating a loss-of-function mutant pap90 . The mutation was characterized at molecular level followed by various experiments to analyze the morphological, physiological and biochemical processes of mutant under control and abiotic stresses. Results: The pap90 mutant showed reduced photosynthesis due to D1 protein instability that subsequently causes inadequate accumulation of thylakoid membrane complexes, especially PSII and decreases PSII functional efficiency. Expression of OsFtsH family genes and proteins were induced in the mutant, which are known to play a key role in D1 protein degradation and turnover. The reduced D1 protein accumulation in the mutant increased the production of reactive oxygen species (ROS). The accumulation of ROS along with the increased activity of antioxidant enzymes and induced expression of stress-associated genes and proteins in pap90 mutant contributed to its water-limited stress tolerance ability. Conclusion: We propose that PAP90 is a key auxiliary protein that interacts with D1 protein and maintains its stability, thereby promoting subsequent assembly of the PSII and associated membrane complexes. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University.)
Databáze:	MEDLINE
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