Dystrophin deficiency impairs vascular structure and function in the canine model of Duchenne muscular dystrophy.

Autor: Kodippili K; Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO, USA., Thorne PK; Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA., Laughlin MH; Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA., Duan D; Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO, USA.; Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA.; Department of Neurology, School of Medicine, University of Missouri, Columbia, MO, USA.; Department of Biomedical, Biological & Chemical Engineering, College of Engineering, University of Missouri, Columbia, MO, USA.
Jazyk: angličtina
Zdroj: The Journal of pathology [J Pathol] 2021 Aug; Vol. 254 (5), pp. 589-605. Date of Electronic Publication: 2021 Jun 14.
DOI: 10.1002/path.5704
Abstrakt: Duchenne muscular dystrophy (DMD) is a muscle-wasting disease caused by dystrophin deficiency. Vascular dysfunction has been suggested as an underlying pathogenic mechanism in DMD. However, this has not been thoroughly studied in a large animal model. Here we investigated structural and functional changes in the vascular smooth muscle and endothelium of the canine DMD model. The expression of dystrophin and endothelial nitric oxide synthase (eNOS), neuronal NOS (nNOS), and the structure and function of the femoral artery from 15 normal and 16 affected adult dogs were evaluated. Full-length dystrophin was detected in the endothelium and smooth muscle in normal but not affected dog arteries. Normal arteries lacked nNOS but expressed eNOS in the endothelium. NOS activity and eNOS expression were reduced in the endothelium of dystrophic dogs. Dystrophin deficiency resulted in structural remodeling of the artery. In affected dogs, the maximum tension induced by vasoconstrictor phenylephrine and endothelin-1 was significantly reduced. In addition, acetylcholine-mediated vasorelaxation was significantly impaired, whereas exogenous nitric oxide-induced vasorelaxation was significantly enhanced. Our results suggest that dystrophin plays a crucial role in maintaining the structure and function of vascular endothelium and smooth muscle in large mammals. Vascular defects may contribute to DMD pathogenesis. © 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
(© 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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