Fibroblast Growth Factor Type 2 (FGF2) Administration Attenuated the Clinical Manifestations of Preeclampsia in a Murine Model Induced by L-NAME.

Autor:	Martinez-Fierro ML; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y C.S, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Hernadez-Delgadillo GP; Laboratorio de Investigacion en Farmacologia, Unidad Academica de Ciencias Quimicas, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Flores-Mendoza JF; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y C.S, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Alvarez-Zuñiga CD; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y C.S, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Diaz-Lozano ML; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y C.S, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Delgado-Enciso I; Department of Molecular Medicine, School of Medicine, University of Colima, Colima, Mexico., Romero-Diaz VJ; Department of Histology, Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Monterrey, Mexico., Lopez-Saucedo A; Health Sciences Area, Universidad Autonoma de Zacatecas, Zacatecas, Mexico., Rodriguez-Sanchez IP; Molecular and Structural Physiology Laboratory, School of Biological Sciences, Autonomous University of Nuevo Leon, Monterrey, Mexico., Marino-Martinez IA; Departamento de Patologia, Facultad de Medicina, Autonomous University of Nuevo Leon, Monterrey, Mexico., Garza-Veloz I; Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y C.S, Universidad Autonoma de Zacatecas, Zacatecas, Mexico.
Jazyk:	angličtina
Zdroj:	Frontiers in pharmacology [Front Pharmacol] 2021 Apr 20; Vol. 12, pp. 663044. Date of Electronic Publication: 2021 Apr 20 (Print Publication: 2021).
DOI:	10.3389/fphar.2021.663044
Abstrakt:	Background: In preeclampsia, a hypertensive disorder of pregnancy, the poor remodeling of spiral arteries leads to placental hypoperfusion and ischemia, provoking generalized maternal endothelial dysfunction and, in severe cases, death. Endothelial and placental remodeling is important for correct pregnancy evolution and is mediated by cytokines and growth factors such as fibroblast growth factor type 2 (FGF2). In this study, we evaluated the effect of human recombinant FGF2 (rhFGF2) administration in a murine model of PE induced by NG-nitro-L-arginine methyl ester (L-NAME) to test if rhFGF2 administration can lessen the clinical manifestations of PE. Methods: Pregnant rats were administrated with 0.9% of NaCl (vehicle), L-NAME (60 mg/kg), FGF2 (666.6 ng/kg), L-NAME+FGF2 or L-NAME + hydralazine (10 mg/kg) from the 10th to 19th days of gestation. Blood pressure (BP), urine protein concentrations and anthropometric values both rat and fetuses were assessed. Histological evaluation of organs from rats delivered by cesarean section was carried out using hematoxylin and eosin staining. Results: A PE-like model was established, and it included phenotypes such as maternal hypertension, proteinuria, and fetal growth delay. Compared to the groups treated with L-NAME, the L-NAME + FGF2 group was similar to vehicle: the BP remained stable and the rats did not develop enhanced proteinuria. Both the fetuses and placentas from rats treated with L-NAME + FGF2 had similar values of weight and size compared with the vehicle. Conclusion: The intravenous administration of rhFGF2 showed beneficial and hypotensive effects, reducing the clinical manifestations of PE in the evaluated model. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Martinez-Fierro, Hernadez-Delgadillo, Flores-Mendoza, Alvarez-Zuñiga, Diaz-Lozano, Delgado-Enciso, Romero-Diaz, Lopez-Saucedo, Rodriguez-Sanchez, Marino-Martinez and Garza-Veloz.)
Databáze:	MEDLINE
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