Role of rhBMP-7, Fibronectin, And Type I Collagen in Dental Implant Osseointegration Process: An Initial Pilot Study on Minipig Animals.

Autor: Schierano G; Department of Surgical Science, C.I.R. Dental School, University of Torino, Via Nizza 230, 10126 Torino, Italy., Canuto RA; Department of Clinical and Biological Sciences, University of Torino, Corso Raffaello 30, 10125 Torino, Italy., Mauthe von Degerfeld M; Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy., Navone R; Department of Medical Science, University of Torino, Via Santena 5, 10126 Torino, Italy., Peirone B; Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy., Preti G; Department of Surgical Science, C.I.R. Dental School, University of Torino, Via Nizza 230, 10126 Torino, Italy., Muzio G; Department of Clinical and Biological Sciences, University of Torino, Corso Raffaello 30, 10125 Torino, Italy.
Jazyk: angličtina
Zdroj: Materials (Basel, Switzerland) [Materials (Basel)] 2021 Apr 24; Vol. 14 (9). Date of Electronic Publication: 2021 Apr 24.
DOI: 10.3390/ma14092185
Abstrakt: Background: The biological factors involved in dental implant osseointegration need to be investigated to improve implant success.
Methods: Twenty-four implants were inserted into the tibias of six minipigs. Bone samples were obtained at 7, 14, and 56 days. Biomolecular analyses evaluated mRNA of BMP-4, -7, Transforming Growth Factor-β2, Interleukin-1β, and Osteocalcin in sites treated with rhBMP-7, Type 1 Collagen, or Fibronectin (FN). Inflammation and osteogenesis were evaluated by histological analyses.
Results: At 7 and 14 days, BMP-4 and BMP-7 increased in the sites prepared with rhBMP-7 and FN. BMP-7 remained greater at 56 days in rhBMP-7 and FN sites. BPM-4 at 7 and 14 days increased in Type 1 Collagen sites; BMP-7 increased from day 14. FN increased the TGF-β2 at all experimental times, whilst the rhBMP-7 only did so up to 7 days. IL-1β increased only in collagen-treated sites from 14 days. Osteocalcin was high in FN-treated sites. Neutrophilic granulocytes characterized the inflammatory infiltrate at 7 days, and mononuclear cells at 14 and 56 days.
Conclusions: This initial pilot study, in a novel way, evidenced that Type 1 Collagen induced inflammation and did not stimulate bone production; conversely FN or rhBMP-7 showed neo-osteogenetic and anti-inflammatory properties when directly added into implant bone site.
Databáze: MEDLINE
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