Identification and characterization of centromeric sequences in Xenopus laevis .

Autor:	Smith OK; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.; Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California 94305, USA., Limouse C; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA., Fryer KA; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.; Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA., Teran NA; Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA., Sundararajan K; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA., Heald R; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720-3200, USA., Straight AF; Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305-5307, USA.
Jazyk:	angličtina
Zdroj:	Genome research [Genome Res] 2021 Jun; Vol. 31 (6), pp. 958-967. Date of Electronic Publication: 2021 Apr 19.
DOI:	10.1101/gr.267781.120
Abstrakt:	Centromeres play an essential function in cell division by specifying the site of kinetochore formation on each chromosome for mitotic spindle attachment. Centromeres are defined epigenetically by the histone H3 variant Centromere Protein A (Cenpa). Cenpa nucleosomes maintain the centromere by designating the site for new Cenpa assembly after dilution by replication. Vertebrate centromeres assemble on tandem arrays of repetitive sequences, but the function of repeat DNA in centromere formation has been challenging to dissect due to the difficulty in manipulating centromeres in cells. Xenopus laevis egg extracts assemble centromeres in vitro, providing a system for studying centromeric DNA functions. However, centromeric sequences in Xenopus laevis have not been extensively characterized. In this study, we combine Cenpa ChIP-seq with a k -mer based analysis approach to identify the Xenopus laevis centromere repeat sequences. By in situ hybridization, we show that Xenopus laevis centromeres contain diverse repeat sequences, and we map the centromere position on each Xenopus laevis chromosome using the distribution of centromere-enriched k -mers. Our identification of Xenopus laevis centromere sequences enables previously unapproachable centromere genomic studies. Our approach should be broadly applicable for the analysis of centromere and other repetitive sequences in any organism. (© 2021 Smith et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu