| Autor: |
Li GR; Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China., Li H; Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China., Lyu Z; Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China., Chen Z; Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China., Wang YG; Department of Internal Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. |
| Jazyk: |
čínština |
| Zdroj: |
Zhonghua xin xue guan bing za zhi [Zhonghua Xin Xue Guan Bing Za Zhi] 2021 Apr 24; Vol. 49 (4), pp. 345-352. |
| DOI: |
10.3760/cma.j.cn112148-20200603-00458 |
| Abstrakt: |
Objective: To investigate the effect of bilateral superior cervical ganglionectomy on cardiac remodeling and function in pressure-overloaded heart failure (HF) mice. Methods: Pressure-overloaded HF mouse model was produced by severe thoracic aorta banding (sTAB). Bilateral superior cervical ganglionectomy (SCGx) was performed 2 weeks after sTAB. Twenty four 6-week-old male C57BL/6 mice were randomized divided into 4 groups ( n =6 each): control group: sham sTAB+sham SCGx; denervated group: sham sTAB+SCGx; HF group: sTAB+sham SCGx; denervated HF group: sTAB+SCGx. Cardiac function was measured by echocardiography at week 0, 1, 2, and 4 after sTAB, respectively. All mice were sacrificed at the end of week 4 and heart tissues were harvested. HE and Masson staining were performed. Immunohistochemical staining (IHC) for tyrosine hydroxylase (TH), adrenergic receptor β1 (AR-β1) and CD68 was performed. Western blot was used to determine the protein expression level of TH, B type natriuretic peptide (BNP), and AR-β1. Results: Left ventricular ejection fraction (LVEF) declined continuously in HF group. LVEF was similar between denervated HF group and control group at various time points ( P >0.05). LVEF was significantly higher in denervated HF group than in HF group at the end of week 4 ( P <0.05). HE staining showed that cross sectional cardiomyocyte area was significantly larger in HF group than in control group and denervated HF group ( P <0.05), which was similar between denervated HF group and control group ( P >0.05). Masson staining showed that fibrosis level was significantly lower in denervated HF group than in HF group ( P <0.05). IHC showed that TH+nerves and CD68 + macrophages were significantly increased in HF mice as compared to control mice ( P <0.05), whereas this change was abolished in denervated HF group. AR-β1 was significantly down-regulated in HF group compared with control group ( P <0.05), which was not affected by denervation ( P >0.05). Western blot demonstrated that the expression level of TH and BNP was significantly higher in HF group compared with the control group ( P <0.05), whereas this difference was diminished in denervated HF group ( P >0.05). Conclusion: Bilateral superior cervical ganglionectomy can reduce sympathetic innervation and macrophage infiltration in pressure overloaded failure heart, thus attenuate cardiac remodeling and improve cardiac function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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