Alteration of cofactor specificity of the acrylyl-CoA reductase from Escherichia coli.
Autor: | Reshetnikov AS; Laboratory of Methylotrophy, Federal Research Center, 'Pushchino Scientific Center for Biological Research, Russian Academy of Sciences' IBPM RAS, Pushchino, Moscow Region, Russia, 142290., But SY; Laboratory of Methylotrophy, Federal Research Center, 'Pushchino Scientific Center for Biological Research, Russian Academy of Sciences' IBPM RAS, Pushchino, Moscow Region, Russia, 142290., Rozova ON; Laboratory of Methylotrophy, Federal Research Center, 'Pushchino Scientific Center for Biological Research, Russian Academy of Sciences' IBPM RAS, Pushchino, Moscow Region, Russia, 142290., Mustakhimov II; Laboratory of Methylotrophy, Federal Research Center, 'Pushchino Scientific Center for Biological Research, Russian Academy of Sciences' IBPM RAS, Pushchino, Moscow Region, Russia, 142290., Khmelenina VN; Laboratory of Methylotrophy, Federal Research Center, 'Pushchino Scientific Center for Biological Research, Russian Academy of Sciences' IBPM RAS, Pushchino, Moscow Region, Russia, 142290. khmelenina@ibpm.pushchino.ru. |
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Jazyk: | angličtina |
Zdroj: | Biotechnology letters [Biotechnol Lett] 2021 Jul; Vol. 43 (7), pp. 1421-1427. Date of Electronic Publication: 2021 Apr 16. |
DOI: | 10.1007/s10529-021-03130-0 |
Abstrakt: | Objectives: Alteration of the cofactor specificity of acrylyl-CoA reductase (AcuI) catalyzing the NAD(P)H-dependent reduction of acrylyl-CoA to propionyl-CoA is often desirable for designing of artificial metabolic pathways of various appointments. Results: Several variants of AcuIs from Escherichia coli K-12 with multiple amino acid substitutions to alter the cofactor preference were obtained by site directed mutagenesis and the modified enzymes as His Conclusions: These results demonstrated a relevance of Asp156 in the interaction of AcuI from E. coli K-12 with NADH as a coenzyme. These findings may provide reference information for shifting coenzyme specificity of acrylyl-CoA reductases. |
Databáze: | MEDLINE |
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