Combined photodynamic therapy with chloroaluminum phthalocyanine and doxorubicin nanoemulsions in breast cancer model.

Autor: Cabral ÁS; Laboratory of Genomic Studies, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil., Leonel ECR; Laboratory of Microscopy and Microanalysis, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil., Candido NM; Laboratory of Genomic Studies, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil., Piva HL; Chemistry Department, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, University of São Paulo; Ribeirão Preto, São Paulo 14040-901, Brazil., de Melo MT; Chemistry Department, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, University of São Paulo; Ribeirão Preto, São Paulo 14040-901, Brazil., Taboga SR; Laboratory of Microscopy and Microanalysis, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil., Rahal P; Laboratory of Genomic Studies, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil., Tedesco AC; Chemistry Department, Center of Nanotechnology and Tissue Engineering - Photobiology and Photomedicine Research Group, University of São Paulo; Ribeirão Preto, São Paulo 14040-901, Brazil., Calmon MF; Laboratory of Genomic Studies, São Paulo State University, São José do Rio Preto, São Paulo 15054-000, Brazil. Electronic address: marilia.calmon@unesp.br.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2021 May; Vol. 218, pp. 112181. Date of Electronic Publication: 2021 Mar 24.
DOI: 10.1016/j.jphotobiol.2021.112181
Abstrakt: Breast cancer is the most common neoplasm among women but thanks to innovative therapies, patients' prognosis has considerably improved. In this aspect, nanotechnology has been applied for cancer therapy aiming to reduce its usual side effects. In this study we aimed to evaluate the effects of nanoemulsions containing photosensitizer and chemotherapeutic agents associated with photodynamic therapy in a breast cancer in vivo model. Our results showed that synergistic treatments in which chloroaluminum phthalocyanine (NE-Pc) administered together with Doxorubicin (Dox) in the presence of laser irradiation (NE-PcDoxo + PDT) led to a reduction of 4 T1 induced breast cancer in mice, decline of tumor VEGF expression, increase in Caspase-3 expression, tissue necrosis and massive decrease in proliferative cells, as shown by Ki67 immunostaining. Furthermore, this associated treatment induced overexpression of apoptotic genes ABL1, CD70, CRADD, FASL, and NME5 and a reduction in expression of anticancer drug target genes CDK2, ERBB2, FIGF, IGF2, PARP4 and PGR. These results validate this treatment as a promising alternative to improve the currently applied anticancer strategies.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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