Proposal to Eliminate Urethane-Treated Positive Control Dose Groups in 26-Week Tg.rasH2 Carcinogenicity Studies.

Autor: Paranjpe MG; 537465Charles River Laboratories, Mattawan, MI, USA., Rudmann D; 537465Charles River Laboratories, Mattawan, MI, USA., Sargeant A; 537465Charles River Laboratories, Mattawan, MI, USA., Morse M; 537465Charles River Laboratories, Mattawan, MI, USA., Yonpiam R; 537465Charles River Laboratories, Mattawan, MI, USA., Bonnette K; 537465Charles River Laboratories, Mattawan, MI, USA., Albretsen J; 537465Charles River Laboratories, Mattawan, MI, USA., Papagiannis C; 537465Charles River Laboratories, Mattawan, MI, USA.
Jazyk: angličtina
Zdroj: International journal of toxicology [Int J Toxicol] 2021 May-Jun; Vol. 40 (3), pp. 207-210. Date of Electronic Publication: 2021 Apr 05.
DOI: 10.1177/10915818211003308
Abstrakt: Short-term (26 weeks) Tg.rasH2 mouse carcinogenicity studies have been conducted as an alternative model to the conventional 2-year mouse carcinogenicity studies, using urethane as a positive control material. In these studies, urethane was used at a dose of 1,000 mg/kg/dose, administered intraperitoneally on days 1, 3, and 5. Urethane consistently produces lung adenomas and carcinomas and hemangiosarcomas of the spleen, proving validity of the assay. We conducted 3 pilot studies at 3 different sites of Charles River Laboratories using a lower dose of urethane (500 mg/kg/dose), administered on days 1, 3, and 5, followed by a 12-week observation period. Our results demonstrate that a lower dose can be used successfully with fewer number of animals per sex to prove the validity of the assay. However, based on our cumulative experience with this model, we propose to eliminate positive control dose groups in future Tg.rasH2 carcinogenicity studies.
Databáze: MEDLINE