Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves.

Autor: Henderson BD; School of Physical Sciences, Dublin City University, Dublin 9, Ireland., Kinahan DJ; School of Mechanical Engineering, Dublin City University, Dublin 9, Ireland.; National Center for Sensor Research, Dublin City University, Dublin 9, Ireland.; I-Form, the SFI Research Centre for Advanced Manufacturing, Dublin City University, Dublin 9, Ireland.; The Water Institute, Dublin City University, Dublin 9, Ireland., Rio J; School of Physical Sciences, Dublin City University, Dublin 9, Ireland., Mishra R; Fraunhofer Project Center, Dublin City University, Dublin 9, Ireland., King D; Fraunhofer Project Center, Dublin City University, Dublin 9, Ireland., Torres-Delgado SM; Institute of Microstructure Technology, Karlsruhe Institute of Technology, 76344 Karlsruhe, Germany., Mager D; Institute of Microstructure Technology, Karlsruhe Institute of Technology, 76344 Karlsruhe, Germany., Korvink JG; Institute of Microstructure Technology, Karlsruhe Institute of Technology, 76344 Karlsruhe, Germany., Ducrée J; School of Physical Sciences, Dublin City University, Dublin 9, Ireland.
Jazyk: angličtina
Zdroj: Biosensors [Biosensors (Basel)] 2021 Mar 06; Vol. 11 (3). Date of Electronic Publication: 2021 Mar 06.
DOI: 10.3390/bios11030073
Abstrakt: Within microfluidic technologies, the centrifugal microfluidic "Lab-on-a-Disc" (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize 'wet bench' laboratory protocols. We present the combination of 'event-triggered dissolvable film valves' with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL -1 and limit of quantitation (LoQ) of 135.87 ng mL -1 .
Databáze: MEDLINE
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