Fiber-Based Electrochemical Biosensors for Monitoring pH and Transient Neurometabolic Lactate.

Autor:	Booth MA; Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K.; Department of Materials, Imperial College London, London SW7 2AZ, U.K.; Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, U.K., Gowers SAN; Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K., Hersey M; Department of Chemistry, University of South Carolina, Columbia, South Carolina 29208, United States., Samper IC; Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K., Park S; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.; Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.; KAIST Institute for Health Science and Technology, Daejeon 34141, Republic of Korea., Anikeeva P; Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States., Hashemi P; Department of Materials, Imperial College London, London SW7 2AZ, U.K.; Department of Chemistry, University of South Carolina, Columbia, South Carolina 29208, United States., Stevens MM; Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K.; Department of Materials, Imperial College London, London SW7 2AZ, U.K.; Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, U.K., Boutelle MG; Department of Bioengineering, Imperial College London, London SW7 2AZ, U.K.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2021 May 04; Vol. 93 (17), pp. 6646-6655. Date of Electronic Publication: 2021 Apr 02.
DOI:	10.1021/acs.analchem.0c05108
Abstrakt:	Developing tools that are able to monitor transient neurochemical dynamics is important to decipher brain chemistry and function. Multifunctional polymer-based fibers have been recently applied to monitor and modulate neural activity. Here, we explore the potential of polymer fibers comprising six graphite-doped electrodes and two microfluidic channels within a flexible polycarbonate body as a platform for sensing pH and neurometabolic lactate. Electrodes were made into potentiometric sensors (responsive to pH) or amperometric sensors (lactate biosensors). The growth of an iridium oxide layer made the fiber electrodes responsive to pH in a physiologically relevant range. Lactate biosensors were fabricated via platinum black growth on the fiber electrode, followed by an enzyme layer, making them responsive to lactate concentration. Lactate fiber biosensors detected transient neurometabolic lactate changes in an in vivo mouse model. Lactate concentration changes were associated with spreading depolarizations, known to be detrimental to the injured brain. Induced waves were identified by a signature lactate concentration change profile and measured as having a speed of ∼2.7 mm/min ( n = 4 waves). Our work highlights the potential applications of fiber-based biosensors for direct monitoring of brain metabolites in the context of injury.
Databáze:	MEDLINE
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