Glucose-induced internalization of the S. cerevisiae galactose permease Gal2 is dependent on phosphorylation and ubiquitination of its aminoterminal cytoplasmic tail.

Autor:	Tamayo Rojas SA; Faculty of Biological Sciences, Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Straße 9, Frankfurt am Main 60438, Germany., Schmidl S; Faculty of Biological Sciences, Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Straße 9, Frankfurt am Main 60438, Germany., Boles E; Faculty of Biological Sciences, Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Straße 9, Frankfurt am Main 60438, Germany., Oreb M; Faculty of Biological Sciences, Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Straße 9, Frankfurt am Main 60438, Germany.
Jazyk:	angličtina
Zdroj:	FEMS yeast research [FEMS Yeast Res] 2021 Apr 07; Vol. 21 (3).
DOI:	10.1093/femsyr/foab019
Abstrakt:	The hexose permease Gal2 of Saccharomyces cerevisiae is expressed only in the presence of its physiological substrate galactose. Glucose tightly represses the GAL2 gene and also induces the clearance of the transporter from the plasma membrane by ubiquitination and subsequent degradation in the vacuole. Although many factors involved in this process, especially those responsible for the upstream signaling, have been elucidated, the mechanisms by which Gal2 is specifically targeted by the ubiquitination machinery have remained elusive. Here, we show that ubiquitination occurs within the N-terminal cytoplasmic tail and that the arrestin-like proteins Bul1 and Rod1 are likely acting as adaptors for docking of the ubiquitin E3-ligase Rsp5. We further demonstrate that phosphorylation on multiple residues within the tail is indispensable for the internalization and possibly represents a primary signal that might trigger the recruitment of arrestins to the transporter. In addition to these new fundamental insights, we describe Gal2 mutants with improved stability in the presence of glucose, which should prove valuable for engineering yeast strains utilizing complex carbohydrate mixtures present in hydrolysates of lignocellulosic or pectin-rich biomass. (© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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