In vivo tumour imaging employing regional delivery of novel gallium radiolabelled polymer composites.

Autor: Stephens RW; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia. ross.stephens@anu.edu.au., Tredwell GD; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia., Bell JL; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia., Knox KJ; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia., Philip LA; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia., Senden TJ; The Biomedical Radiochemistry Laboratory, Department of Applied Mathematics, Research School of Physics, Australian National University, Canberra, ACT, Australia., Tapner MJ; Sirtex Medical Ltd, Sydney, Australia., Bickley SA; Sirtex Medical Ltd, Sydney, Australia., Tanudji MR; Sirtex Medical Ltd, Sydney, Australia., Jones SK; Sirtex Medical Ltd, Sydney, Australia.
Jazyk: angličtina
Zdroj: Biomaterials research [Biomater Res] 2021 Mar 31; Vol. 25 (1), pp. 7. Date of Electronic Publication: 2021 Mar 31.
DOI: 10.1186/s40824-021-00210-0
Abstrakt: Background: Understanding the regional vascular delivery of particles to tumour sites is a prerequisite for developing new diagnostic and therapeutic composites for treatment of oncology patients. We describe a novel imageable 67 Ga-radiolabelled polymer composite that is biocompatible in an animal tumour model and can be used for preclinical imaging investigations of the transit of different sized particles through arterial networks of normal and tumour-bearing organs.
Results: Radiolabelling of polymer microspheres with 67 Ga was achieved using a simple mix and wash method, with tannic acid as an immobilising agent. Final in vitro binding yields after autoclaving averaged 94.7%. In vivo stability of the composite was demonstrated in New Zealand white rabbits by intravenous administration, and intrahepatic artery instillations were made in normal and VX2 tumour implanted rabbit livers. Stability of radiolabel was sufficient for rabbit lung and liver imaging over at least 3 hours and 1 hour respectively, with lung retention of radiolabel over 91%, and retention in both normal and VX2 implanted livers of over 95%. SPECT-CT imaging of anaesthetised animals and planar imaging of excised livers showed visible accumulation of radiolabel in tumours. Importantly, microsphere administration and complete liver dispersal was more easily achieved with 8 μm diameter MS than with 30 μm MS, and the smaller microspheres provided more distinct and localised tumour imaging.
Conclusion: This method of producing 67 Ga-radiolabelled polymer microspheres is suitable for SPECT-CT imaging of the regional vascular delivery of microspheres to tumour sites in animal models. Sharper distinction of model tumours from normal liver was obtained with smaller MS, and tumour resolution may be further improved by the use of 68 Ga instead of 67 Ga, to enable PET imaging.
Databáze: MEDLINE
Externí odkaz: