[Effect of electroacupuncture on neurovascular unit and Wnt/β-catenin signaling in rats with cerebral ischemia].

Autor:	Li GD; Graduate School of Anhui University of Chinese Medicine, Hefei 230012, China., Li XX; Graduate School of Anhui University of Chinese Medicine, Hefei 230012, China., Dong JJ; The Affiliated Hospital of Institute of Neurology, Anhui University of Chinese Medicine, Hefei 230061., Wu Y; Hefei Institute of Substances of Chinese Academy of Sciences, Hefei 230031., Han YS; The Affiliated Hospital of Institute of Neurology, Anhui University of Chinese Medicine, Hefei 230061; Institute of Neurology of Anhui University of Chinese Medicine, Hefei 230038.
Jazyk:	čínština
Zdroj:	Zhen ci yan jiu = Acupuncture research [Zhen Ci Yan Jiu] 2021 Feb 25; Vol. 46 (2), pp. 87-94.
DOI:	10.13702/j.1000-0607.200819
Abstrakt:	Objective: To observe the effect of electroacupuncture (EA) at "Baihui" (GV20), "Shuigou" (GV26), etc. on the expressions of vascular endothelial growth factor (VEGF), collagen fibrillary acidic protein (GFAP), neuronal nucleus antigen(NeuN), β-catenin and Axin2 protein and mRNA in rats with cerebral ischemia (CI), so as to explore its mechanism underlying improvement of ischemic stroke. Methods: A total of 108 male SD rats were randomly divided into control, model and EA groups, which were further divided into 7 d, 14 d and 21 d subgroups, with 12 rats in each group. The CI model was established by occlusion of the middle cerebral artery. EA (2 Hz/100 Hz, 2-4 V) was applied to GV20, GV26, bilateral "Sanyinjiao" (SP6) and bilateral "Neiguan" (PC6) for 30 min, once daily (except Sundays) for 21 days at most. The neurological deficit score was evaluated according to Longa's methods. The cerebral infarction state was assessed by using a magnetic resonance T2 imaging system. The expression levels of neurovascular markers as VEGF,GFAP and NeuN, and β-catenin and Axin2 protein and mRNA in the ischemic brain tissue were detected by using immunohistochemistry and quantitative real-time PCR, respectively. Results: After modeling, the neurological deficit score and cerebral infarction size were significantly increased (P<0.01), and the expression of NeuN and Axin2 proteins and mRNAs were significantly and gradually decreased with time (day 7, 14 and 21) (P<0.01), whereas the expression levels of VEGF, GFAP, β-catenin proteins and mRNAs were significantly increased on day 7, 14 and 21 in the model group relevant to the control group (P<0.01). Compared with the model group, the neurological deficit score, cerebral infarction size and the expressions of Axin2 protein and mRNA were significantly decreased on day 7, 14 and 21 (P<0.01), whereas the expression levels of VEGF, GFAP and NeuN and β-catenin proteins and mRNAs were considerably up-regulated in the EA group on day 7, 14 and 21 (P<0.01). Conclusion: EA can protect the neurovascular units from injury, reduce the volume of cerebral infarction and improve the symptoms of neurological deficit in cerebral ischemic rats, which may be related to its effects in up-regulating β-catenin expression and in down-regulating Axin2 expression to further activate classical Wnt/ β-catenin signal pathway.
Databáze:	MEDLINE
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