| Autor: |
Chernova ON; I. I. Mechnikov North-Western State Medical University, Ministry of Health if the Russian Federation, St. Petersburg, Russia. olgachernova92@yandex.ru.; St. Petersburg Medical-Social Institute, St. Petersburg, Russia. olgachernova92@yandex.ru., Mavlikeev MO; I. I. Mechnikov North-Western State Medical University, Ministry of Health if the Russian Federation, St. Petersburg, Russia.; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Kiyasov AP; Kazan (Volga region) Federal University, Kazan, Republic of Tatarstan, Russia., Bozo IY; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Deev RV; I. I. Mechnikov North-Western State Medical University, Ministry of Health if the Russian Federation, St. Petersburg, Russia.; Institute of Human Stem Cells, Moscow, Russia. |
| Abstrakt: |
The study assessed reactivity of stromal-vascular skeletal muscle differons to acute chemical injury. Dysferlin-deficient Bla/J mice and the wild-type С57BL/6 mice were intramuscularly injected with 100 μl of 0.5% procaine solution. The middle segment of gastrocnemius muscle was taken on postsurgery days 2, 4, 10, and 14 for routine histological examination. To evaluate proliferation and vascularization, the paraffin sections were stained immunohistochemically with antibodies to α-smooth muscle actin and Ki-67. The connective tissue was stained according to Mallory. The study revealed diminished proliferative activity of stromal-vascular differons and decreased vascular density in muscles of Bla/J mice. Thus, mutations in the DYSF gene coding dysferlin down-regulate the reparation processes in all differons of skeletal muscle. |
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