Antenatal Dexamethasone Treatment Induces Sex-dependent Upregulation of NTPDase1/CD39 and Ecto-5'-nucleotidase/CD73 in the Rat Fetal Brain.

Autor: Manojlovic-Stojanoski M; Institute for Biological Research, 'Siniša Stanković'- National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Lavrnja I; Institute for Biological Research, 'Siniša Stanković'- National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Stevanovic I; Medical Faculty of Military Medical Academy, Institute of Medical Research Belgrade, Belgrade, Serbia., Trifunovic S; Institute for Biological Research, 'Siniša Stanković'- National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Ristic N; Institute for Biological Research, 'Siniša Stanković'- National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Nestorovic N; Institute for Biological Research, 'Siniša Stanković'- National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia., Sévigny J; Département de Microbiologie-Infectiologie et d'immunologie, Faculté de Médecine, Université Laval, Québec, Canada.; Centre de recherche du CHU de Québec - Université Laval, Québec, Canada., Nedeljkovic N; Department for General Physiology and Biophysics, Faculty of Biology, University of Belgrade, Belgrade, Serbia., Laketa D; Department for General Physiology and Biophysics, Faculty of Biology, University of Belgrade, Belgrade, Serbia. danijela@bio.bg.ac.rs.
Jazyk: angličtina
Zdroj: Cellular and molecular neurobiology [Cell Mol Neurobiol] 2022 Aug; Vol. 42 (6), pp. 1965-1981. Date of Electronic Publication: 2021 Mar 24.
DOI: 10.1007/s10571-021-01081-8
Abstrakt: Dexamethasone (DEX) is frequently used to treat women at risk of preterm delivery, but although indispensable for the completion of organ maturation in the fetus, antenatal DEX treatment may exert adverse sex-dimorphic neurodevelopmental effects. Literature findings implicated oxidative stress in adverse effects of DEX treatment. Purinergic signaling is involved in neurodevelopment and controlled by ectonucleotidases, among which in the brain the most abundant are ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1/CD39) and ecto-5'-nucleotidase (e5'NT/CD73), which jointly dephosphorylate ATP to adenosine. They are also involved in cell adhesion and migration, processes integral to brain development. Upregulation of CD39 and CD73 after DEX treatment was reported in adult rat hippocampus. We investigated the effects of maternal DEX treatment on CD39 and CD73 expression and enzymatic activity in the rat fetal brain of both sexes, in the context of oxidative status of the brain tissue. Fetuses were obtained at embryonic day (ED) 21, from Wistar rat dams treated with 0.5 mg DEX/kg/day, at ED 16, 17, and 18, and brains were processed and used for further analysis. Sex-specific increase in CD39 and CD73 expression and in the corresponding enzyme activities was induced in the brain of antenatally DEX-treated fetuses, more prominently in males. The oxidative stress induction after antenatal DEX treatment was confirmed in both sexes, although showing a slight bias in males. Due to the involvement of purinergic system in crucial neurodevelopmental processes, future investigations are needed to determine the role of these observed changes in the adverse effects of antenatal DEX treatment.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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