Chimeric antigen-guiding extracellular vesicles eliminate antigen-specific Th2 cells in subjects with food allergy.

Autor:	Zhang YY; Department of Respirology & Allergy, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.; Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China., Mo LH; Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China., Yang G; Department of Otolaryngology, Longgang Central Hospital, Shenzhen, China., Liu JQ; Department of Allergy, Longgang ENT Hospital & Shenzhen Key Laboratory of ENT, Institute of ENT, Shenzhen, China., Liu ZQ; Department of Allergy, Longgang ENT Hospital & Shenzhen Key Laboratory of ENT, Institute of ENT, Shenzhen, China., Yang LT; Department of Respirology & Allergy, Third Affiliated Hospital of Shenzhen University, Shenzhen, China., Ran PX; Department of Respirology, First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China., Liu ZG; Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China., Yang PC; Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.; Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Shenzhen, China.
Jazyk:	angličtina
Zdroj:	The World Allergy Organization journal [World Allergy Organ J] 2021 Feb 24; Vol. 14 (3), pp. 100522. Date of Electronic Publication: 2021 Feb 24 (Print Publication: 2021).
DOI:	10.1016/j.waojou.2021.100522
Abstrakt:	Background: Antigen (Ag)-specific T helper (Th)2 cells play a central role in food allergy (FA) pathogenesis. Methods can be used to eliminate Ag-specific Th2 cells that are currently lacking. This study aims to eliminate the Ag-specific Th2 cells with a novel nanoparticle, the mEV (modified extracellular vesicles, that carry a chimeric antigen peptide, MHC II and caspase 3) in a murine FA model. Methods: mEVs were generated by exposing dendritic cells (DC) to ovalbumin (OVA, a specific Ag) and recombinant caspase 3 (Casp3) in the culture overnight. Exosomes were purified from culture supernatant by the magnetic antibody approach. A murine FA model was developed with OVA as the specific Ag. Results: Purified mEVs had the molecular markers of extracellular vesicle, CD81, CD63, and CD9, cleaved Casp3 and MHC II/OVA complexes. mEVs specifically bound to the surface of Ag-specific CD4 + T cells, induced Ag-specific CD4 + T cell apoptosis both in vitro and in vivo as well as increased regulatory T cells in the intestinal tissues. Administration of mEV efficiently suppressed experimental FA. Conclusions: mEVs carry Ag/MHC II complexes and Casp3, that can induce Ag-specific Th2 cell apoptosis. Administration of mEV can efficiently suppress experimental FA. The results suggest that the mEVs have the translational potential to be used in the treatment of FA and other allergic diseases. Competing Interests: None to declare. (© 2021 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu