Development of a nanotechnology-based approach for capturing and detecting nucleic acids by using flow cytometry.

Autor: Robles-Remacho A; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja S/n, 18071, Granada, Spain; Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada/University of Granada, Avenida Del Conocimiento, S/n, 18016, Granada, Spain., Luque-González MA; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja S/n, 18071, Granada, Spain; Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada/University of Granada, Avenida Del Conocimiento, S/n, 18016, Granada, Spain., González-Casín RA; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain., Cano-Cortés MV; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja S/n, 18071, Granada, Spain; Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada/University of Granada, Avenida Del Conocimiento, S/n, 18016, Granada, Spain., Lopez-Delgado FJ; DestiNA Genomica S.L, PTS Granada, Avenida de La Innovación 1, Edificio BIC, 18100, Armilla, Granada, Spain., Guardia-Monteagudo JJ; DestiNA Genomica S.L, PTS Granada, Avenida de La Innovación 1, Edificio BIC, 18100, Armilla, Granada, Spain., Antonio Fara M; DestiNA Genomica S.L, PTS Granada, Avenida de La Innovación 1, Edificio BIC, 18100, Armilla, Granada, Spain., Sánchez-Martín RM; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja S/n, 18071, Granada, Spain; Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada/University of Granada, Avenida Del Conocimiento, S/n, 18016, Granada, Spain. Electronic address: rmsanchez@go.ugr.es., Díaz-Mochón JJ; GENYO. Centre for Genomics and Oncological Research: Pfizer / University of Granada / Andalusian Regional Government, PTS Granada, Avenida de La Ilustracion, 114, 18016, Granada, Spain; Department of Medicinal and Organic Chemistry, School of Pharmacy, University of Granada, Campus Cartuja S/n, 18071, Granada, Spain; Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada/University of Granada, Avenida Del Conocimiento, S/n, 18016, Granada, Spain. Electronic address: juanjose.diaz@genyo.es.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122092. Date of Electronic Publication: 2021 Jan 09.
DOI: 10.1016/j.talanta.2021.122092
Abstrakt: Nucleic acid-based molecular diagnosis has gained special importance for the detection and early diagnosis of genetic diseases as well as for the control of infectious disease outbreaks. The development of systems that allow for the detection and analysis of nucleic acids in a low-cost and easy-to-use way is of great importance. In this context, we present a combination of a nanotechnology-based approach with the already validated dynamic chemical labeling (DCL) technology, capable of reading nucleic acids with single-base resolution. This system allows for the detection of biotinylated molecular products followed by simple detection using a standard flow cytometer, a widely used platform in clinical and molecular laboratories, and therefore, is easy to implement. This proof-of-concept assay has been developed to detect mutations in KRAS codon 12, as these mutations are highly important in cancer development and cancer treatments.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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