Fabrication of a sensitive colorimetric nanosensor for determination of cysteine in human serum and urine samples based on magnetic-sulfur, nitrogen graphene quantum dots as a selective platform and Au nanoparticles.

Autor: Afsharipour R; Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran., Dadfarnia S; Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran. Electronic address: sdadfarnia@yazd.ac.ir., Haji Shabani AM; Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran. Electronic address: hshabani@yazd.ac.ir., Kazemi E; Environmental and Bio-Analytical Laboratories, Department of Chemistry, Sharif University of Technology, Tehran, Iran., Pedrini A; Department of Chemistry, Life Science and Environmental Sustainability, University of Parma, Parco Area Delle Scienze 17/A, 43124 Parma, Italy., Verucchi R; IMEM-CNR, Institute of Materials for Electronics and Magnetism, Trento Unit C/o Fondazione Bruno Kessler, Via Alla Cascata 56/C, Povo, Trento IT-38123, Italy.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2021 May 01; Vol. 226, pp. 122055. Date of Electronic Publication: 2021 Jan 09.
DOI: 10.1016/j.talanta.2020.122055
Abstrakt: A novel colorimetric nanosensor is reported for the selective and sensitive determination of cysteine using magnetic-sulfur, nitrogen graphene quantum dots (Fe 3 O 4 /S, N-GQDs), and gold nanoparticles (Au NPs). Thus, S, N-GQDs was firstly immobilized on Fe 3 O 4 nanoparticles through its magnetization in the presence of Fe 3+ in the alkali solution. The prepared Fe 3 O 4 /S, N-GQDs were dispersed in cysteine solution resulting in its quick adsorption on the surface of the Fe 3 O 4 /S, N-GQDs through hydrogen bonding interaction. Then, Au NPs solution was added to this mixture that after a short time, the color of Au NPs changed from red to blue, the intensity of surface plasmon resonance peak of Au NPs at 530 nm decreased, and a new peak at a higher wavelength of 680 nm appeared. The effective parameters on cysteine quantification were optimized via response surface methodology using the central composite design. Under optimum conditions, the absorbance ratio (A 680 /A 530 ) has a linear proportionality with cysteine concentration in the range of 0.04-1.20 μmol L -1 with a limit of detection of 0.009 μmol L -1 . The fabrication of the reported nanosensor is simple, fast, and is capable of efficient quantification of ultra traces of cysteine in human serum and urine real samples.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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