[Effects of a high-fat diet on the lipid profile of oocytes in mice].

Autor:	Brusentsev EY; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Chuyko EA; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, Novosibirsk State University, Novosibirsk, Russia., Okotrub KA; Institute of Automation and Electrometry of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Igonina TN; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Rozhkova IN; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Ragaeva DS; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Ranneva SV; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, Novosibirsk State University, Novosibirsk, Russia., Naprimerov VA; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, Novosibirsk State Agrarian University, Novosibirsk, Russia., Amstislavsky SY; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, Novosibirsk State University, Novosibirsk, Russia.
Jazyk:	ruština
Zdroj:	Vavilovskii zhurnal genetiki i selektsii [Vavilovskii Zhurnal Genet Selektsii] 2020 Aug; Vol. 24 (5), pp. 533-538.
DOI:	10.18699/VJ20.645
Abstrakt:	There are evidences that obese women exhibit a detrimental oocyte quality. However, it remains unclear how this change is associated with obesity, indirectly - or directly through a change in the content and/or composition of lipids in oocytes. The aim of this work was to study effects of a high-fat diet applied to female donor mice on the amount and qualitative composition of lipids of immature and in vivo matured oocytes. A high-fat diet caused larger body weight in female mice compared with the control ( p < 0.001; 44.77 ± 1.46 and 35.22 ± 1.57, respectively), and increased the blood levels of cholesterol ( p < 0.05; 2.06 ± 0.10 and 1.78 ± 0.10, respectively) and triglycerides ( p < 0.05; 2.13 ± 0.23 and 1.49 ± 0.21, respectively). At the same time, this diet does not affect the level of unsaturation of lipids in immature (0.207 ± 0.004 in the experiment and 0.206 ± 0.002 in the control) and matured oocytes (0.212 ± 0.005 in the experiment and 0.211 ± 0.003 in the control). Total lipid content increased during in vivo maturation of mouse oocytes. The amount of lipids was greater in mature oocytes in the experimental group compared to the control ( p < 0.01; 8.15 ± 0.37 and 5.83 ± 0.14, respectively). An increase in intracellular lipid amount during oocyte maturation was revealed both after a standard diet ( p < 0.05; 4.72 ± 0.48 and 5.83 ± 0.14, respectively) and after a fat-rich diet ( p < 0.001; 3.45 ± 0.62 and 8.15 ± 0.37, respectively). Thus, during in vivo oocyte maturation in mice the content of intracellular lipids enhanced, the high-fat diet aggravated this dynamics of lipid increase during in vivo maturation of oocytes. (Copyright © AUTHORS, 2018.)
Databáze:	MEDLINE
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