Actin cables and comet tails organize mitochondrial networks in mitosis.

Autor: Moore AS; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Coscia SM; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Cell and Molecular Biology Graduate Group, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA., Simpson CL; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Department of Dermatology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA., Ortega FE; Department of Biochemistry, Stanford University, Stanford, CA, USA., Wait EC; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Heddleston JM; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Nirschl JJ; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA., Obara CJ; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Guedes-Dias P; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany., Boecker CA; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA., Chew TL; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Theriot JA; Department of Biology, University of Washington, Seattle, WA, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA., Lippincott-Schwartz J; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA, USA., Holzbaur ELF; Department of Physiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. holzbaur@pennmedicine.upenn.edu.; The Pennsylvania Muscle Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. holzbaur@pennmedicine.upenn.edu.; Cell and Molecular Biology Graduate Group, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. holzbaur@pennmedicine.upenn.edu.
Jazyk: angličtina
Zdroj: Nature [Nature] 2021 Mar; Vol. 591 (7851), pp. 659-664. Date of Electronic Publication: 2021 Mar 03.
DOI: 10.1038/s41586-021-03309-5
Abstrakt: Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. Whereas the mechanisms coordinating the segregation of the genome are well known, the processes that ensure organelle segregation between daughter cells remain less well understood 1 . Here we identify multiple actin assemblies with distinct but complementary roles in mitochondrial organization and inheritance in mitosis. First, we find a dense meshwork of subcortical actin cables assembled throughout the mitotic cytoplasm. This network scaffolds the endoplasmic reticulum and organizes three-dimensional mitochondrial positioning to ensure the equal segregation of mitochondrial mass at cytokinesis. Second, we identify a dynamic wave of actin filaments reversibly assembling on the surface of mitochondria during mitosis. Mitochondria sampled by this wave are enveloped within actin clouds that can spontaneously break symmetry to form elongated comet tails. Mitochondrial comet tails promote randomly directed bursts of movement that shuffle mitochondrial position within the mother cell to randomize inheritance of healthy and damaged mitochondria between daughter cells. Thus, parallel mechanisms mediated by the actin cytoskeleton ensure both equal and random inheritance of mitochondria in symmetrically dividing cells.
Databáze: MEDLINE
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