| Autor: |
Mesquita PHC; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Lamb DA; Department of Nutrition, Dietetics and Hospitality Management, Auburn University, Auburn, AL 36849, USA., Godwin JS; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Osburn SC; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Ruple BA; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Moore JH; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Vann CG; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Huggins KW; Department of Nutrition, Dietetics and Hospitality Management, Auburn University, Auburn, AL 36849, USA., Fruge AD; Department of Nutrition, Dietetics and Hospitality Management, Auburn University, Auburn, AL 36849, USA., Young KC; School of Kinesiology, Auburn University, Auburn, AL 36849, USA.; Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn, AL 36832, USA., Kavazis AN; School of Kinesiology, Auburn University, Auburn, AL 36849, USA., Roberts MD; School of Kinesiology, Auburn University, Auburn, AL 36849, USA.; Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn, AL 36832, USA. |
| Abstrakt: |
The aim of this study was to investigate the effects of resistance training (RT) on the redox status of skeletal muscle in older adults. Thirteen males aged 64 ± 9 years performed full-body RT 2x/week for 6 weeks. Muscle biopsies were obtained from the vastus lateralis prior to and following RT. The mRNA, protein, and enzymatic activity levels of various endogenous antioxidants were determined. In addition, skeletal muscle 4-hydroxynonenal and protein carbonyls were determined as markers of oxidative damage. Protein levels of heat shock proteins (HSPs) were also quantified. RT increased mRNA levels of all assayed antioxidant genes, albeit protein levels either did not change or decreased. RT increased total antioxidant capacity, catalase, and glutathione reductase activities, and decreased glutathione peroxidase activity. Lipid peroxidation also decreased and HSP60 protein increased following RT. In summary, 6 weeks of RT decreased oxidative damage and increased antioxidant enzyme activities. Our results suggest the older adult responses to RT involve multi-level (transcriptional, post-transcriptional, and post-translational) control of the redox status of skeletal muscle. |
