Mitochondrial function is controlled by melatonin and its metabolites in vitro in human melanoma cells.

Autor: Bilska B; Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Kraków, Poland., Schedel F; Department of Dermatology, University of Münster, Münster, Germany., Piotrowska A; Department of Histology, Medical University of Gdańsk, Gdańsk, Poland., Stefan J; Department of Oncology, Nicolaus Copernicus University Medical College, Bydgoszcz, Poland.; Department of Dermatology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA., Zmijewski M; Department of Histology, Medical University of Gdańsk, Gdańsk, Poland., Pyza E; Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Kraków, Poland., Reiter RJ; Department of Cellular and Structural Biology, UT Health, San Antonio, TX, USA., Steinbrink K; Department of Dermatology, University of Münster, Münster, Germany., Slominski AT; Department of Dermatology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA.; Pathology and Laboratory Medicine Service, VA Medical Center, Birmingham, AL, USA., Tulic MK; Université Côte d'Azur, INSERM U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), Nice, France., Kleszczyński K; Department of Dermatology, University of Münster, Münster, Germany.
Jazyk: angličtina
Zdroj: Journal of pineal research [J Pineal Res] 2021 Apr; Vol. 70 (3), pp. e12728. Date of Electronic Publication: 2021 Mar 14.
DOI: 10.1111/jpi.12728
Abstrakt: Melanoma is a leading cause of cancer deaths worldwide. Although immunotherapy has revolutionized the treatment for some patients, resistance towards therapy and unwanted side effects remain a problem for numerous individuals. Broad anti-cancer activities of melatonin are recognized; however, additional investigations still need to be elucidated. Herein, using various human melanoma cell models, we explore in vitro the new insights into the regulation of melanoma by melatonin and its metabolites which possess, on the other side, high safety profiles and biological meaningful. In this study, using melanotic (MNT-1) and amelanotic (A375, G361, Sk-Mel-28) melanoma cell lines, the comparative oncostatic responses, the impact on melanin content (for melanotic MNT-1 melanoma cells) as well as the mitochondrial function controlled by melatonin, its precursor (serotonin), a kynuric (N 1 -acetyl-N 2 -formyl-5-methoxykynuramine, AFMK) and indolic pathway (6-hydroxymelatonin, 6(OH)MEL and 5-methoxytryptamine, 5-MT) metabolites were assessed. Namely, significant disturbances were observed in bioenergetics as follows: (i) uncoupling of oxidative phosphorylation (OXPHOS), (ii) attenuation of glycolysis, (iii) dissipation of mitochondrial transmembrane potential (mtΔΨ) accompanied by (iv) massive generation of reactive oxygen species (ROS), and (v) decrease of glucose uptake. Collectively, these results together with previously published reports provide a new biological potential and make an imperative to consider using melatonin or its metabolites for complementary future treatments of melanoma-affected patients; however, these associations should be additionally investigated in clinical setting.
(© 2021 The Authors. Journal of Pineal Research published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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