| Autor: |
Pekov SI; Skolkovo Institute of Science and Technology, Moscow, Russia; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia., Sorokin AA; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia., Kuzin AA; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia., Bocharov KV; Semenov Federal Center of Chemical Physic of RAS, Moscow, Russia., Bormotov DS; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia., Shivalin AS; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia., Shurkhay VA; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia; N.N. Burdenko National Medical Research Center of Neurosurgery, Moscow, Russia., Potapov AA; N.N. Burdenko National Medical Research Center of Neurosurgery, Moscow, Russia., Nikolaev EN; Skolkovo Institute of Science and Technology, Moscow, Russia., Popov IA; Moscow Institute of Physics and Technology (National Research University), Moscow, Russia. |
| Abstrakt: |
Significant metabolism alteration is accompanying the cell malignization process. Energy metabolism disturbance leads to the activation of de novo synthesis and beta-oxidation processes of lipids and fatty acids in a cancer cell, which becomes an indicator of pathological processes inside the cell. The majority of studies dealing with lipid metabolism alterations in glial tumors are performed using the cell lines in vitro or animal models. However, such conditions do not entirely represent the physiological conditions of cell growth or possible cells natural variability. This work presents the results of the data obtained by applying ambient mass spectrometry to human glioblastoma multiform tissues. By analyzing a relatively large cohort of primary and secondary glioblastoma samples, we identify the alterations in cells lipid composition, which accompanied the development of grade IV brain tumors. We demonstrate that primary glioblastomas, as well as ones developed from astrocytomas, are enriched with mono- and diunsaturated phosphatidylcholines (PC 26:1, 30:2, 32:1, 32:2, 34:1, 34:2). Simultaneously, the saturated and polyunsaturated phosphatidylcholines and phosphatidylethanolamines decrease. These alterations are obviously linked to the availability of the polyunsaturated fatty acids and activation of the de novo lipid synthesis and beta-oxidation pathways under the anaerobic conditions in the tumor core. |
