Tracing uptake and translocation of phosphorus in wheat using oxygen isotopes and mathematical modelling.

Autor: Bauke SL; Institute of Crop Science and Resource Conservation (INRES) - Soil Science and Soil Ecology, University of Bonn, Nussallee 13, Bonn, 53115, Germany., Schnepf A; Institute for Bio- and Geosciences - IBG-3: Agrosphere, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, Jülich, 52425, Germany., von Sperber C; Institute of Crop Science and Resource Conservation (INRES) - Soil Science and Soil Ecology, University of Bonn, Nussallee 13, Bonn, 53115, Germany.; Department of Geography, McGill University, 805 Sherbrooke St. W., Montreal, QC, H3A 0B9, Canada., Orlowski N; Institute for Landscape Ecology and Resources Management (ILR), Research Centre for BioSystems, Land Use and Nutrition (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, Giessen, 35392, Germany.; Chair of Hydrology, University of Freiburg, Friedrichstraße 39, Freiburg, 79098, Germany., Lewandowski H; Institute for Bio- and Geosciences - IBG-3: Agrosphere, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, Jülich, 52425, Germany., Selzner T; Institute of Crop Science and Resource Conservation (INRES) - Soil Science and Soil Ecology, University of Bonn, Nussallee 13, Bonn, 53115, Germany., Tamburini F; Institute of Agricultural Sciences, ETH Zürich, Lindau, 8315, Switzerland., Amelung W; Institute of Crop Science and Resource Conservation (INRES) - Soil Science and Soil Ecology, University of Bonn, Nussallee 13, Bonn, 53115, Germany.; Institute for Bio- and Geosciences - IBG-3: Agrosphere, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, Jülich, 52425, Germany.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Jun; Vol. 230 (5), pp. 1883-1895. Date of Electronic Publication: 2021 Apr 05.
DOI: 10.1111/nph.17307
Abstrakt: Understanding P uptake in soil-plant systems requires suitable P tracers. The stable oxygen isotope ratio in phosphate (expressed as δ 18 O P ) is an alternative to radioactive labelling, but the degree to which plants preserve the δ 18 O P value of the P source is unclear. We hypothesised that the source signal will be preserved in roots rather than shoots. In soil and hydroponic experiments with spring wheat (Triticum aestivum), we replaced irrigation water by 18 O-labelled water for up to 10 d. We extracted plant inorganic phosphates with trichloroacetic acid (TCA), assessed temporal dynamics of δ 18 O TCA-P values after changing to 18 O-labelled water and combined the results with a mathematical model. Within 1 wk, full equilibration of δ 18 O TCA-P values with the isotope value of the water in the growth medium occurred in shoots but not in roots. Model results further indicated that root δ 18 O TCA-P values were affected by back transport of phosphate from shoots to roots, with a greater contribution of source P at higher temperatures when back transport was reduced. Root δ 18 O TCA-P partially preserved the source signal, providing an indicator of P uptake sources. This now needs to be tested extensively for different species, soil and climate conditions to enable application in future ecosystem studies.
(© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.)
Databáze: MEDLINE
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