The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.

Autor: McCann JR; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Bihlmeyer NA; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Roche K; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Catherine C; Department of Pediatrics, Duke University, Durham, North Carolina, USA., Jawahar J; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Kwee LC; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Younge NE; Department of Pediatrics, Duke University, Durham, North Carolina, USA., Silverman J; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Ilkayeva O; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Sarria C; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Zizzi A; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Wootton J; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Poppe L; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Anderson P; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Arlotto M; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Wei Z; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA., Granek JA; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.; Duke Microbiome Center, Duke University Durham, North Carolina, USA., Valdivia RH; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.; Duke Microbiome Center, Duke University Durham, North Carolina, USA., David LA; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.; Duke Microbiome Center, Duke University Durham, North Carolina, USA., Dressman HK; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.; Duke Microbiome Center, Duke University Durham, North Carolina, USA., Newgard CB; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA., Shah SH; Duke Molecular Physiology Institute and Sarah W. Stedman Nutrition and Metabolism Center, Duke University, Durham, North Carolina, USA.; Duke Clinical Research Institute, Duke University, Durham, North Carolina, USA., Seed PC; Ann & Robert H. Lurie Children's Hospital of Chicago, Northwestern University Feinberg School of Medicine, Stanley Manne Children's Research Institute, Northwestern University Medical Center, Chicago, Illinois, USA., Rawls JF; Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA.; Duke Microbiome Center, Duke University Durham, North Carolina, USA., Armstrong SC; Department of Pediatrics, Duke University, Durham, North Carolina, USA.; Duke Clinical Research Institute, Duke University, Durham, North Carolina, USA.
Jazyk: angličtina
Zdroj: Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2021 Mar; Vol. 29 (3), pp. 569-578.
DOI: 10.1002/oby.23081
Abstrakt: Objective: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification.
Methods: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented.
Results: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid-related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group.
Conclusions: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.
(© 2021 The Obesity Society.)
Databáze: MEDLINE
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