Ex vivo tumor necrosis factor-alpha response of blood leukocytes in Danish Holstein-Friesian cows stimulated by Gram-positive and Gram-negative bacteria isolated from mastitic milk.

Autor: Khatun M; Aarhus University, Faculty of Science and Technology, Department of Animal Science, Blichers Allé 20, P.O. Box 50, DK-8300, Tjele, Denmark. Electronic address: monarahman24@bau.edu.bd., Damgaard BM; Aarhus University, Faculty of Science and Technology, Department of Animal Science, Blichers Allé 20, P.O. Box 50, DK-8300, Tjele, Denmark. Electronic address: BirtheM.Damgaard@agrsci.dk., Andersen JB; Aarhus University, Faculty of Science and Technology, Department of Animal Science, Blichers Allé 20, P.O. Box 50, DK-8300, Tjele, Denmark. Electronic address: jba@anis.au.dk., Røntved CM; Aarhus University, Faculty of Science and Technology, Department of Animal Science, Blichers Allé 20, P.O. Box 50, DK-8300, Tjele, Denmark; Christine Røntved, CMR On-site RD, Graverhusvej 53, Langholt, 9310, Vodskov, Denmark. Electronic address: cmr@cmr-on-site.dk.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2021 Apr; Vol. 234, pp. 110204. Date of Electronic Publication: 2021 Feb 12.
DOI: 10.1016/j.vetimm.2021.110204
Abstrakt: A whole blood stimulation assay was used to investigate the effects of parity, number of weeks after calving and Gram-positive and Gram-negative bacteria on the ex vivo TNF-α responsiveness of Danish Holstein-Friesian cows of first to third lactation (n = 28). Blood samples were collected in weeks 2, 3, 5 and 8 after parturition and stimulated with Escherichia coli LPS (10 μg/mL), Staphylococcus aureus peptidoglycan (PGN, 10 μg/mL) and dead Escherichia coli, Streptococcus uberis, Staphylococcus aureus, and Streptococcus dysgalactiae at a concentration of 2.5 × 10 6 /mL. The antibiotic polymyxin-B (100 μg/mL) was added to the Gram-positive bacteria to avoid the influence of environmental endotoxin by ELISA test. Overall, parity had no effect, whereas number of weeks after calving altered the TNF-α responsiveness of the majority of the stimulants. Ex vivo, Gram-positive bacteria always resulted in a higher TNF-α response than Gram-negative bacteria with large differences within the individual cows. High correlations were found within the Gram-negative stimulants panel (r = 0.83) and within the Gram-positive (r = 0.81 to 0.86) stimulants panel except PGN. The higher TNF-α responsiveness by Gram-positive bacteria is in agreement with in vitro studies in human but in contrast to the in vivo TNF-α responsiveness in bovine udder.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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