Improvements in Gene Editing Technology Boost Its Applications in Livestock.
| Autor: | Perisse IV; Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States., Fan Z; Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States., Singina GN; L.K. Ernst Federal Research Center for Animal Husbandry, Podolsk, Russia., White KL; Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States., Polejaeva IA; Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT, United States. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in genetics [Front Genet] 2021 Jan 08; Vol. 11, pp. 614688. Date of Electronic Publication: 2021 Jan 08 (Print Publication: 2020). |
| DOI: | 10.3389/fgene.2020.614688 |
| Abstrakt: | Accelerated development of novel CRISPR/Cas9-based genome editing techniques provides a feasible approach to introduce a variety of precise modifications in the mammalian genome, including introduction of multiple edits simultaneously, efficient insertion of long DNA sequences into specific targeted loci as well as performing nucleotide transitions and transversions. Thus, the CRISPR/Cas9 tool has become the method of choice for introducing genome alterations in livestock species. The list of new CRISPR/Cas9-based genome editing tools is constantly expanding. Here, we discuss the methods developed to improve efficiency and specificity of gene editing tools as well as approaches that can be employed for gene regulation, base editing, and epigenetic modifications. Additionally, advantages and disadvantages of two primary methods used for the production of gene-edited farm animals: somatic cell nuclear transfer (SCNT or cloning) and zygote manipulations will be discussed. Furthermore, we will review agricultural and biomedical applications of gene editing technology. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Perisse, Fan, Singina, White and Polejaeva.) |
| Databáze: | MEDLINE |
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