| Autor: |
Maliano MR; Department of Plant Pathology, University of California, Davis, CA 95616, U.S.A., Melgarejo TA; Department of Plant Pathology, University of California, Davis, CA 95616, U.S.A., Rojas MR; Department of Plant Pathology, University of California, Davis, CA 95616, U.S.A., Barboza N; Centro de Investigación en Biología Celular y Molecular, Escuela de Tecnología de Alimentos, Centro Nacional en Ciencia y Tecnología de Alimentos, Universidad de Costa Rica, 2060 San José, Costa Rica., Gilbertson RL; Department of Plant Pathology, University of California, Davis, CA 95616, U.S.A. |
| Jazyk: |
angličtina |
| Zdroj: |
Plant disease [Plant Dis] 2021 Oct; Vol. 105 (10), pp. 3162-3170. Date of Electronic Publication: 2021 Nov 07. |
| DOI: |
10.1094/PDIS-08-20-1759-RE |
| Abstrakt: |
Since the early 1990s, squash production in Costa Rica has been affected by a whitefly-transmitted disease characterized by stunting and yellow mottling of leaves. The squash yellow mottle disease (SYMoD) was shown to be associated with a bipartite begomovirus, originally named squash yellow mild mottle virus (SYMMoV). It was subsequently established that SYMMoV is a strain of melon chlorotic leaf curl virus (MCLCuV), a bipartite begomovirus that causes a chlorotic leaf curl disease of melons in Guatemala. In the present study, the complete sequences of the DNA-A and DNA-B components of a new isolate of the strain MCLCuV-Costa Rica (MCLCuV-CR) were determined. Comparisons of full-length DNA-A sequences revealed 97% identity with a previously characterized isolate of MCLCuV-CR and identities of 90 to 91% with those of isolates of the strain MCLCuV-Guatemala (MCLCuV-GT), which is below or at the current begomovirus species demarcation threshold of 91%. A more extensive analysis of the MCLCuV-CR and -GT sequences revealed substantial divergence in both components and different histories of recombination for the DNA-A components. The cloned full-length DNA-A and DNA-B components of this new MCLCuV-CR isolate were infectious and induced SYMoD in a range of squashes and in pumpkin, thereby fulfilling Koch's postulates for this disease. However, in contrast to MCLCuV-GT, MCLCuV-CR induced mild symptoms in watermelon and no symptoms in melon and cucumber. Taken together, our results indicate that MCLCuV-CR and -GT have substantially diverged, genetically and biologically, and have evolved to cause distinct diseases of different cucurbit crops. Taxonomically, these viruses are at the strain/species boundary, but retain the designation as strains of Melon chlorotic leaf curl virus under current International Committee on Taxonomy guidelines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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