Validation of N95 Filtering Facepiece Respirator Decontamination Methods Available at a Large University Hospital.

Autor:	Wigginton KR; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Arts PJ; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Clack HL; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Fitzsimmons WJ; Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Michigan, USA., Gamba M; Department of Aerospace Engineering, University of Michigan, Ann Arbor, Michigan, USA., Harrison KR; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., LeBar W; Department of Pathology, Clinical Microbiology, University of Michigan Health System, Ann Arbor, Michigan, USA., Lauring AS; Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Michigan, USA., Li L; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Roberts WW; Department of Urology, University of Michigan Health System, Ann Arbor, Michigan, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA., Rockey NC; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Torreblanca J; Department of Pathology, Clinical Microbiology, University of Michigan Health System, Ann Arbor, Michigan, USA., Young C; Department of Pathology, Clinical Microbiology, University of Michigan Health System, Ann Arbor, Michigan, USA., Anderegg LG; Department of Physics, Harvard University, Cambridge, Massachusetts, USA.; Harvard-MIT Center for Ultracold Atoms, Cambridge, Massachusetts, USA., Cohn AM; Department of Industrial & Operations Engineering, University of Michigan, Ann Arbor, Michigan, USA., Doyle JM; Department of Physics, Harvard University, Cambridge, Massachusetts, USA.; Harvard-MIT Center for Ultracold Atoms, Cambridge, Massachusetts, USA., Meisenhelder CM; Department of Physics, Harvard University, Cambridge, Massachusetts, USA., Raskin L; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Love NG; Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA., Kaye KS; Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Michigan, USA.
Jazyk:	angličtina
Zdroj:	Open forum infectious diseases [Open Forum Infect Dis] 2020 Dec 15; Vol. 8 (2), pp. ofaa610. Date of Electronic Publication: 2020 Dec 15 (Print Publication: 2021).
DOI:	10.1093/ofid/ofaa610
Abstrakt:	Background: Due to unprecedented shortages in N95 filtering facepiece respirators, healthcare systems have explored N95 reprocessing. No single, full-scale reprocessing publication has reported an evaluation including multiple viruses, bacteria, and fungi along with respirator filtration and fit. Methods: We explored reprocessing methods using new 3M 1860 N95 respirators, including moist (50%-75% relative humidity [RH]) heat (80-82°C for 30 minutes), ethylene oxide (EtO), pulsed xenon UV-C (UV-PX), hydrogen peroxide gas plasma (HPGP), and hydrogen peroxide vapor (HPV). Respirator samples were analyzed using 4 viruses (MS2, phi6, influenza A virus [IAV], murine hepatitis virus [MHV)]), 3 bacteria ( Escherichia coli , Staphylococcus aureus , Geobacillus stearothermophilus spores, and vegetative bacteria), and Aspergillus niger . Different application media were tested. Decontaminated respirators were evaluated for filtration integrity and fit. Results: Heat with moderate RH most effectively inactivated virus, resulting in reductions of >6.6-log 10 MS2, >6.7-log 10 Phi6, >2.7-log 10 MHV, and >3.9-log 10 IAV and prokaryotes, except for G stearothermohphilus . Hydrogen peroxide vapor was moderately effective at inactivating tested viruses, resulting in 1.5- to >4-log 10 observable inactivation. Staphylococcus aureus inactivation by HPV was limited. Filtration efficiency and proper fit were maintained after 5 cycles of heat with moderate RH and HPV. Although it was effective at decontamination, HPGP resulted in decreased filtration efficiency, and EtO treatment raised toxicity concerns. Observed virus inactivation varied depending upon the application media used. Conclusions: Both moist heat and HPV are scalable N95 reprocessing options because they achieve high levels of biological indicator inactivation while maintaining respirator fit and integrity. (© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
Databáze:	MEDLINE
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