Chemokine-Induced PBMC and Subsequent MSC Migration Toward Decellularized Heart Valve Tissue.

Autor: VeDepo MC; Jacot Laboratory for Pediatric Regenerative Medicine, Department of Bioengineering, University of Colorado Anschutz Medical Campus, 12705 E. Montview Ave., Suite 100, Aurora, CO, 80045, USA. mitchell.vedepo@cuanschutz.edu., Flores K; Jacot Laboratory for Pediatric Regenerative Medicine, Department of Bioengineering, University of Colorado Anschutz Medical Campus, 12705 E. Montview Ave., Suite 100, Aurora, CO, 80045, USA., Jacot JG; Jacot Laboratory for Pediatric Regenerative Medicine, Department of Bioengineering, University of Colorado Anschutz Medical Campus, 12705 E. Montview Ave., Suite 100, Aurora, CO, 80045, USA.; Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Jazyk: angličtina
Zdroj: Cardiovascular engineering and technology [Cardiovasc Eng Technol] 2021 Jun; Vol. 12 (3), pp. 325-338. Date of Electronic Publication: 2021 Feb 09.
DOI: 10.1007/s13239-021-00522-1
Abstrakt: Purpose: Enhancing the recellularization of a decellularized heart valve in situ may lead to an improved or ideal heart valve replacement. A promising approach is leveraging the immune response for inflammation-mediated recellularization. However, this mechanism has not been previously demonstrated in vitro.
Methods: This study investigated loading the chemokine MCP-1 into decellularized porcine heart valve tissue and measured the migration of human peripheral blood mononuclear cells (PBMCs) and mesenchymal stem cells (MSCs) toward the chemokine loaded valve tissue.
Results: The results of this study demonstrate that MCP-1-loaded tissues increase PBMC migration compared to non-loaded tissues. Additionally, we demonstrate MCP-1-loaded tissues that have recruited PBMCs lead to increased migration of MSCs compared to decellularized tissue alone.
Conclusion: The results of this study provide evidence for the inflammation-mediated recellularization mechanism. Furthermore, the results support the use of such an approach for enhancing the recellularization of a decellularized heart valve.
Databáze: MEDLINE
Externí odkaz: