Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2.

Autor: Wrobel AG; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. antoni.wrobel@crick.ac.uk., Benton DJ; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. donald.benton@crick.ac.uk., Xu P; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK.; Precision Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China., Calder LJ; Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT, London, UK., Borg A; Structural Biology Science Technology Platform, Francis Crick Institute, NW1 1AT, London, UK., Roustan C; Structural Biology Science Technology Platform, Francis Crick Institute, NW1 1AT, London, UK., Martin SR; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK., Rosenthal PB; Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT, London, UK., Skehel JJ; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK., Gamblin SJ; Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. steve.gamblin@crick.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Feb 05; Vol. 12 (1), pp. 837. Date of Electronic Publication: 2021 Feb 05.
DOI: 10.1038/s41467-021-21006-9
Abstrakt: Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2.
Databáze: MEDLINE
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