[Persistence of Epstein-Barr virus antigens in gastric cancer: characteristic of inflammatory cellular reactions in tumor].

Autor:	Danilova NV; Medical Research and Educational Center of the Lomonosov Moscow State University, Moscow, Russia., Mikhailov IA; Medical Research and Educational Center of the Lomonosov Moscow State University, Moscow, Russia., Oleynikova NA; Medical Research and Educational Center of the Lomonosov Moscow State University, Moscow, Russia., Malkov PG; Medical Research and Educational Center of the Lomonosov Moscow State University, Moscow, Russia.; Russian Medical Academy of Continuous Professional Education of the Ministry of Health of Russia, Moscow, Russia., Chayka AV; P.A. Herzen Moscow Oncology Research Institute - Branch of the National Medical Radiology Research Center of the Ministry of Health of Russia, Moscow, Russia., Khomyakov VM; P.A. Herzen Moscow Oncology Research Institute - Branch of the National Medical Radiology Research Center of the Ministry of Health of Russia, Moscow, Russia., Kakotkin VV; Medical Research and Educational Center of the Lomonosov Moscow State University, Moscow, Russia., Yudin MY; Separate subdivision «SM-Medica» of «SM-Clinic» LLC, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Arkhiv patologii [Arkh Patol] 2021; Vol. 83 (1), pp. 18-24.
DOI:	10.17116/patol20218301118
Abstrakt:	The Aim of the Study: Is to establish the relationship between the persistence of viral antigens of the Epstein-Barr virus (EBV) and the cellular composition of the immune microenvironment of tumor tissue and the mucous membrane of peritumoral area in gastric cancer. Material and Methods: We used samples of surgical material from 55 patients with a verified diagnosis of gastric cancer. The expression of CD4, CD8, CD68, CD1a and LMP-1 was assessed. The results were assessed by the morphometric method. We selected three fields of view (magnification x200) in tumor tissue and in peritumoral areas separately and counted an absolute number of cells with positive staining with further calculation of the average number of cells and the median. Results: LMP-1-negative tumors with LMP-1 expression in epithelium of peritumoral area were characterized by the highest density of CD4+ lymphocyte infiltration in the central part of the tumor; the highest density of CD8+ lymphocyte infiltration in the mucous membrane of peritumoral area ( p =0.0190); the highest density of infiltration by macrophages in the mucous membrane of peritumoral area ( p =0.2492); the highest density of infiltration by CD1a+ cells in the mucous membrane of peritumoral area ( p =0.1503). The highest density of infiltration with CD1a+ cells was characteristic for LMP-1-positive and LMP-1-negative tumors ( p =0.0813). The persistence of the LMP-1 viral antigen in the glandular epithelium of the peritumoral area in our sample does not have a statistically significant effect on the prognosis of the disease (RR=1.7718; p =0.0885) but there is a tendency towards a negative predictive value. Conclusion: High density of infiltration of glandular epithelium of peritumoral area with the expression of LMP-1 by CD4+ and CD8+ lymphocytes is most likely associated with the activation of the cellular immune response and may be one of the signs of the persistence of viral antigens. It was shown for the first time that the phenomenon of persistence of the LMP-1 viral antigen is characterized by a trend towards negative predictive value for patients with gastric cancer.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu