Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer.

Autor: Castilho ML; Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil; Laboratório de Bionanotecnologia, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil., Jesus VPS; Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil., Vieira PFA; Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil., Hewitt KC; Department of Physics and Atmospheric Science, Dalhousie University, 6310 Coburg Road, Halifax, Nova Scotia B3H 4R2, Canada., Raniero L; Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo 12244-000, Brazil. Electronic address: lraniero@univap.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2021 Mar; Vol. 33, pp. 102186. Date of Electronic Publication: 2021 Jan 23.
DOI: 10.1016/j.pdpdt.2021.102186
Abstrakt: To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 μg/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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