Whole planarian chromosome squash.
| Autor: | Barghouth PG; Department of Molecular & Cell Biology, University of California, Merced, Merced, CA, USA.; Quantitative and Systems Biology Graduate Program, University of California, Merced, Merced, CA, USA., Oviedo NJ; Department of Molecular & Cell Biology, University of California, Merced, Merced, CA, USA.; Quantitative and Systems Biology Graduate Program, University of California, Merced, Merced, CA, USA.; Health Sciences Research Institute, University of California, Merced, Merced, CA, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Jan 09; Vol. 2 (1), pp. 100257. Date of Electronic Publication: 2021 Jan 09 (Print Publication: 2021). |
| DOI: | 10.1016/j.xpro.2020.100257 |
| Abstrakt: | Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals. For complete details on the use and execution of this protocol, please refer to Peiris et al. (2016). Competing Interests: The authors declare no competing interests. (© 2020 The Author(s).) |
| Databáze: | MEDLINE |
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