Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells.

Autor: Jiang W; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Wong J; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Tan HX; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Kelly HG; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Whitney PG; WHO Collaborating Centre for Reference and Research On Influenza, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Barr I; WHO Collaborating Centre for Reference and Research On Influenza, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia., Layton DS; CSIRO Health and Biosecurity, Australian Animal Health Laboratories, Geelong, VIC, Australia., Kent SJ; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia.; Melbourne Sexual Health Clinic and Infectious Diseases Department, Alfred Hospital, Monash University Central Clinical School, Carlton, VIC, Australia.; ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Melbourne, Australia., Wheatley AK; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia. a.wheatley@unimelb.edu.au., Juno JA; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, Australia. jennifer.juno@unimelb.edu.au.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Jan 21; Vol. 11 (1), pp. 1864. Date of Electronic Publication: 2021 Jan 21.
DOI: 10.1038/s41598-021-81389-z
Abstrakt: The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre-clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret-reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.
Databáze: MEDLINE
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