The effects of voluntary complex and regular wheel running exercises on the levels of 8-oxoguanine DNA glycosylase, semaphorin 3B, H2O2, and apoptosis in the hippocampus of diabetic rats.

Autor: Fazelzadeh M; Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran., Afzalpour ME; Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran., Fallah Mohammadi Z; Faculty of Sport Sciences, Department of Exercise Physiology, University of Mazandaran, Babolsar, Iran., Falah Mohammadi H; Faculty of Natural Sciences, Department of Biology, Ulm University, Baden-Württemberg, Germany.
Jazyk: angličtina
Zdroj: Brain and behavior [Brain Behav] 2021 Mar; Vol. 11 (3), pp. e01988. Date of Electronic Publication: 2021 Jan 20.
DOI: 10.1002/brb3.1988
Abstrakt: Purpose: One of the most frequent complications associated with diabetes mellitus is apoptosis within the brain which can lead to cognitive disorders. Exercise is considered the best non-pharmacological approach to reduce the severity and extent of cell death through poorly-understood mechanisms. The aim of this study was to investigate the effects of voluntary complex and regular wheel running on the levels of 8-oxoguanine DNA glycosylase (OGG 1 ), semaphorin 3B (sema3B), hydrogen peroxide (H 2 O 2 ), and apoptosis in the hippocampus of diabetic rats.
Methods: 48 Wistar male rats were randomly divided into 6 groups: healthy control (C), diabetes control (D), regular wheel running + diabetes (RWD), complex wheel running + diabetes (CWD), healthy regular wheel running (RW), and healthy complex wheel running (CW). The diabetic rat model was produced by intraperitoneal injection of streptozotocin (STZ). The protocol encompassed a 4-week voluntary running training regimen on regular and complex wheel running apparatus. The rats were sacrificed 48 hr after the last training session. To measure the protein concentrations within the hippocampus, ELISA has been utilized. One-way ANOVA was used to compare the groups.
Results: There were no significant differences in OGG1 protein levels between the groups. H 2 O 2 level in the D group was significantly higher than the C group (p = .002), while this in RWD and CWD groups was considerably lower than the D group (p = .002 and p = .003, respectively). In the D group, the levels of apoptosis and Sema3B were significantly (p = .001 and p = .007, respectively) higher than C, RWD (p = .001, p = .0001, respectively), and CWD groups (p = .001, p = .006, respectively). Nevertheless, there were not any significant differences between RWD and CWD groups.
Conclusion: The increased levels of Sema3B, H2O2, and apoptosis within the hippocampus associated with diabetes could be noticeably restored by both types of voluntary wheel running protocols.
(© 2021 The Authors. Brain and Behavior published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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